Oligonucleotide analysis is a critical aspect of molecular biology and therapeutic development, demanding robust and versatile chromatographic techniques for comprehensive characterization. Several modes of chromatography can be employed to characterize these biomolecules, but when and how to best use each mode can be challenging. This webinar aims to explore how various chromatographic techniques can be synergistically employed to achieve comprehensive oligonucleotide characterization. Aspects of ion-pair reversed phase chromatography (IP-RPC), hydrophilic interaction liquid chromatography (HILIC), ion exchange chromatography (IEC), and size exclusion chromatography (SEC) will be discussed and how best to employ each mode in oligonucleotide characterization. Several application examples will be utilized to showcase how each of these modes of chromatography can be best utilized to fully resolve and understand the composition of samples containing oligonucleotides.
At the conclusion of the webinar, the audience members will:
- Understand the strengths and weaknesses of each mode of chromatography in characterizing oligonucleotides.
- Be familiar with key method parameters to adjust when fine-tuning oligonucleotide chromatographic assays.
- Gain an appreciation for when to use each mode of chromatography when analyzing oligonucleotides.
Speakers
Cory E. Muraco
Merck
Biomolecule Workflows Manager
Cory Muraco is the Biomolecule Workflows Manager at MilliporeSigma, Bellefonte, USA. Cory joined the company in 2013, starting in the Chemical Standards division, developing and validating analytical methods for testing the purity of certified reference materials using GC and LC methods. In 2022, Cory assumed his present role, where he is tasked with supporting the design, development, and execution of the marketing and R&D strategies around MilliporeSigma’s bioanalytical strategy.
Andrea Krumm
Tosoh Bioscience GmbH
Product Manager, Analytical Columns
Andrea Krumm studied biotechnology before gaining a PhD in Cell and Cancer Biology. After four years as an applications specialist for optical analytical devices used in academic and pharmaceutical research, she joined Tosoh Bioscience GmbH in 2020 as a product manager for analytical columns. Andrea is responsible for gathering customer requirements, application areas and addressing these with new and existing products of the analytical columns line.
Analytical chemistry
- Large molecule HPLC
Duration:1h
Language:English
Session 1:presented November 8, 2023
Session 2:presented November 8, 2023
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