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53822-U

Ascentis^® Express C18 (2.7 μm) HPLC Column

Name: Ascentis<SUP>®</SUP> Express C18 (2.7 μm) HPLC Column
Brand: SUPELCO

L × I.D. 5 cm × 2.1 mm, pk of 1 ea

Synonym(s):

Core-shell (SPP) Fused Core C18 HPLC column

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About This Item

UNSPSC Code:

41115700

NACRES:

SB.52

eCl@ss:

32110501

L × i.d.:

5 cm × 2.1 mm

Particle size:

2.7 μm

Matrix active group:

C18 (octadecyl) phase

Pore size:

90 Å

Matrix:

Fused-Core particle platform, superficially porous particle

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Properties

material

stainless steel column

Quality Level

100

agency

suitable for USP L1

product line

Ascentis^®

feature

endcapped

packaging

pk of 1 ea

manufacturer/tradename

Ascentis^®

parameter

60 °C temp. range, 600 bar max. pressure (9000 psi)

technique(s)

HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable

L × I.D.

5 cm × 2.1 mm

surface area

135 m²/g

impurities

<5 ppm metals

matrix

Fused-Core particle platform, superficially porous particle

matrix active group

C18 (octadecyl) phase

particle size

2.7 μm

pore size

90 Å

operating pH

2-9

application(s)

food and beverages

separation technique

reversed phase

Description

General description

Ascentis Express HPLC columns, through the use of Fused-Core^® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC^® (or other ultra high pressure system) users, as well as conventional HPLC users.
Visit the Ascentis Express home page for more information on this new column technology.

Application

HPLC Analysis of Capsaicins in Chili Powder on Ascentis^® Express C18, 2.7 μm
UHPLC/MS Analysis of Corn Oil on Ascentis^® Express C30 Compared to Ascentis^® Express C18
UHPLC Analysis of Paracetamol and Impurities on Ascentis^® Express C18, 2.7 μm
HPLC Analysis of Parabens on Ascentis^®Express C18, 2.7 μm

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Fused-Core is a registered trademark of Advanced Materials Technology, Inc.

UPLC is a registered trademark of Waters

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pictograms

GHS07

signalword

Warning

hcodes

H317

pcodes

P280

Hazard Classifications

Skin Sens. 1

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Protocols

Fast LC-MS-MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3

We presents a robust method for analyzing 25-hydroxyvitamin D3 and 25-hydroxyvitamin D2 in serum using a multiplexed LC-MS-MS system.

Articles

Derivatization Agents for LC/MS – An Improved Detection of Estradiol with ESI-MS

Improved LC/MS Detection of Estradiol using 4-(Dimethylamino) benzoyl chloride (DMABC) derivatization agent relative to dansyl chloride.

Derivatization and Separation of Aliphatic Amines

Derivatization of Aliphatic Amines - Results from the Supelco Ascentis Express column show higher peak resolution, and improved peak shapes than typical chromatograms.

The Importance of Filtration in Optimizing (U)HPLC and LC-MS Performance

Learn how filter selection and sample and mobile phase filtration impact HPLC back pressure and column lifetime.

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Untargeted profiling of Glycyrrhiza glabra extract with comprehensive two-dimensional liquid chromatography-mass spectrometry using multi-segmented shift gradients in the second dimension: Expanding the metabolic coverage.

Yong Foo Wong et al.

Electrophoresis (2018-02-08)

Metabolic profiling of Glycyrrhiza glabra using comprehensive two-dimensional liquid chromatography (LC × LC) coupled with photodiode array (PDA) and mass spectrometry (MS) detection is described. The separation was conducted under reversed-phase conditions, using a combination of first dimension (1 D) 150 mm microbore

Development and Validation of an LC-MS-MS Method for Determination of Simvastatin and Simvastatin Acid in Human Plasma: Application to a Pharmacokinetic Study.

Pankaj Partani et al.

Journal of chromatographic science, 54(8), 1385-1396 (2016-05-27)

A liquid chromatography-tandem mass spectrometry method was developed and validated for the simultaneous determination of simvastatin (SV) and simvastatin acid (SVA) in human plasma. To improve assay sensitivity and achieve simultaneous analysis, SVA monitored in (-)ESI (electrospray ionization) mode within

Determination of tropane alkaloids atropine and scopolamine by liquid chromatography-mass spectrometry in plant organs of Datura species.

Silvia Jakabová et al.

Journal of chromatography. A, 1232, 295-301 (2012-03-07)

Hyoscyamine (atropine) and scopolamine are the predominant tropane alkaloids in the Datura genus, occurring in all plant organs. The assessment of the alkaloid content of various plant parts is essential from the viewpoint of medical use, but also as a

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Global Trade Item Number

SKU	GTIN
53822-U	04061838534279