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Merck
CN

E3138

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse

clone F4, ascites fluid

Synonym(s):

Anti-EGFR

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About This Item

UNSPSC Code:
51111800
NACRES:
NA.41
MDL number:
MFCD00162282
Conjugate:
unconjugated
Clone:
F4, monoclonal
Application:
ARR, ELISA (i), IHC (f), IHC (p), IP, WB
Citations:
28

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

F4, monoclonal

contains

15 mM sodium azide

species reactivity

human

packaging

antibody small pack of 25 μL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunohistochemistry (frozen sections): suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: 1:2,000

isotype

IgG1

UniProt accession no.

P00533

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... EGFR(1956)

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General description

Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kD, which spans the membranes of a wide range of normal and malignant epithelial cells. EGF-R have been identified in sarcomas, gliomas, breast, bladder, lung and gynecological tumors. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour.

Immunogen

synthetically produced residues 985-996 of the human EGF receptor.

Application

Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in:
  • western blotting
  • immunoprecipitation
  • immunocytochemistry
  • immunohistochemical staining

Biochem/physiol Actions

EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.
Epidermal growth factor receptor (EGF-R) is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus.
This antibody recognizes specifically an intracellular domain of the EGF receptor.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

动植物来源生物产品
常规特殊物品
This item has

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Certificates of Analysis (COA)

Lot/Batch Number
0000525928
0000525928
0000527511
0000527511
0000508428

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Changes in expression of epidermal growth factor receptors by anterior pituitary cells during the estrous cycle: cyclic expression by gonadotropes
Armstrong J and Childs GV
Endocrinology, 138(5), 1903-1908 (1997)
Localization of the epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in the bovine testis
Kassab M, et al.
Journal of Molecular Histology, 38(3), 207-214 (2007)
T Cerny et al.
British journal of cancer, 54(2), 265-269 (1986-08-01)
Epidermal growth factor receptor (EGF-R) expression was assessed in 63 lung tumour samples with a monoclonal antibody (EGF-R1) by indirect immunoperoxidase staining on cryostat sections. All 15 small cell lung cancer samples were negative whereas over 80% of the 48
Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway
Steen H, et al.
The Journal of Biological Chemistry, 277(2), 1031-1039 (2002)
Erik Samén et al.
Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 54(10), 1804-1811 (2013-09-05)
Several tracers have been evaluated as probes for noninvasive epidermal growth factor receptor (EGFR) quantification with PET. One of the most promising candidates is the (11)C-labeled analog of the EGFR tyrosine kinase inhibitor PD153035. However, previous in vitro studies indicated
View All Related Papers

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Data Sheet - E3138

Datasheet

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