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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
F4, monoclonal
包含
15 mM sodium azide
种属反应性
human
包装
antibody small pack of 25 μL
技术
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1:2,000
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... EGFR(1956)
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相关类别
一般描述
Monoclonal Anti-Epidermal Growth Factor (EGF) Receptor (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The receptor for epidermal growth factor is an integral cell membrane protein of 170 kD, which spans the membranes of a wide range of normal and malignant epithelial cells. EGF-R have been identified in sarcomas, gliomas, breast, bladder, lung and gynecological tumors. The EGF-R has a half-life of approximately 10 hours in human fibroblasts, but in the presence of EGF this value is reduced to about 1 hour.
特异性
This antibody recognizes specifically an intracellular domain of the EGF receptor.
免疫原
synthetically produced residues 985-996 of the human EGF receptor.
应用
Monoclonal Anti-Epidermal Growth Factor Receptor antibody produced in mouse has been used in:
- western blotting
- immunoprecipitation
- immunocytochemistry
- immunohistochemical staining
生化/生理作用
EGFR is a transmembrane glycoprotein that binds to the EGF family. It is a pivotal regulator of cell proliferation, growth, differentiation and DNA repair after radiation-induced damage. Mutations in the gene encoding EGFR are associated with a number of malignancies including lung cancer and colorectal cancer.
Epidermal growth factor receptor (EGF-R) is a tyrosine-specific protein kinase with the capacity to phosphorylate tyrosine residues located near its carboxy-terminus.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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相关产品
产品编号
说明
价格
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Localization of the epidermal growth factor (EGF) and epidermal growth factor receptor (EGFR) in the bovine testis
Journal of Molecular Histology, 38(3), 207-214 (2007)
Changes in expression of epidermal growth factor receptors by anterior pituitary cells during the estrous cycle: cyclic expression by gonadotropes
Endocrinology, 138(5), 1903-1908 (1997)
Tyrosine phosphorylation mapping of the epidermal growth factor receptor signaling pathway
The Journal of Biological Chemistry, 277(2), 1031-1039 (2002)
British journal of cancer, 54(2), 265-269 (1986-08-01)
Epidermal growth factor receptor (EGF-R) expression was assessed in 63 lung tumour samples with a monoclonal antibody (EGF-R1) by indirect immunoperoxidase staining on cryostat sections. All 15 small cell lung cancer samples were negative whereas over 80% of the 48
Hyaluronan-induced masking of ErbB2 and CD44-enhanced trastuzumab internalisation in trastuzumab resistant breast cancer
European Journal of Cancer, 43(16), 2423-2433 (2007)
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