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安全信息

SCC150

Sigma-Aldrich

16HBE14o-人源支气管上皮细胞系

Human

别名:

16HBE, 16-HBE, 16HBEo-, 16-HBEo, 16-HBE14o

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About This Item

UNSPSC代码:
41106514
eCl@ss:
32011203
NACRES:
NA.81

产品名称

16HBE14o-人源支气管上皮细胞系, 16HBE14o- human bronchial epithelial cell line is widely used to model barrier function of the airway epithelium and to study respiratory ion transport as well as the function of CFTR.

生物来源

human

技术

cell based assay: suitable
cell culture | mammalian: suitable

一般描述

16HBE14o-是一种人支气管上皮细胞系，从1岁的男性心肺患者中分离出来，并用复制起点缺陷型SV40质粒（pSVori-）永生化。 该细胞系保留了正常分化的支气管上皮细胞的特征，包括鹅卵石形态，细胞角蛋白表达，形成紧密连接的能力和定向离子转运（1）。 当与空气/液体界面一起生长时，可以检测到纤毛。与大多数其他呼吸细胞系相反，16HBE14o-表达高水平的囊性纤维化跨膜电导调节剂(CFTR)mRNA和蛋白质（1）。 CFTR的表达与多种细胞（包括16HE14O-上皮细胞）中依赖cAMP的Cl-电导相关。

细胞系描述

上皮细胞

应用

16HBE14O-人支气管上皮细胞系已用于表达环状RNA（circRNA）。它也已用于脂质组学分析中，以与囊性纤维化细胞系进行比较。

质量

• 每小瓶含有≥ 1X106个活细胞。
•通过PCR检测细胞，并通过Charles River动物诊断服务的Human Essential CLEAR面板评估，对HPV-16、HPV-18、甲型、乙型、丙型肝炎和HIV-1 & 2型病毒呈阴性。
•细胞对支原体污染呈阴性。
•通过STR分析对每批细胞进行基因分型，以验证细胞系的唯一身份。

储存及稳定性

储存在液氮中。在初始解冻后，细胞可以培养至少10代，而不会显著影响细胞标志物的表达和功能。

其他说明

根据产品文件中详述的“学术使用协议”条款，本产品预期仅用于销售和销售给学术机构，以供内部学术研究使用。有关任何其他用途的信息，请联系licensing@emdmillipore.com。

免责声明

仅供研究使用在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

高风险级别生物产品-Merck

分析证书(COA)

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Pflugers Archiv : European journal of physiology, 428(5-6), 597-603 (1994-10-01)
The present study was performed to examine Ca(2+)-dependent and cell-swelling-induced ion conductances in a polarized bronchial epithelial cell line (16HBE14o-). Whole-cell currents were measured in fast and slow whole-cell patch-clamp experiments in cells grown either on filters or on coated
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16HBE14o- human bronchial epithelial cells used to model respiratory epithelium for the research of cystic fibrosis, viral pulmonary pathology (SARS-CoV), asthma, COPD, effects of smoking and air pollution. See over 5k publications.

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