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HSTCMAG-28SK

Millipore

MILLIPLEX®人类高灵敏度T细胞小组-免疫学多重测定

Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

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别名:
Human high-sensitivity cytokine multiplex kit, Luminex® human high-sensitivity cytokine immunoassay, Millipore human high-sensitivity cytokine panel
UNSPSC代码:
12161503
eCl@ss:
32161000
NACRES:
NA.47

质量水平

200

种属反应性

human

制造商/商品名称

Milliplex®

assay range

accuracy: 98-107%
sensitivity: 0.11-8.17 pg/mL
(Overnight Incubation minDC)
standard curve range: 0.18-750 pg/mL
(IL-6)
standard curve range: 0.24-1,000 pg/mL
(IL-13)
standard curve range: 0.24-1,000 pg/mL
(IL-21)
standard curve range: 0.31-1,250 pg/mL
(IL-8)
standard curve range: 0.31-1,250 pg/mL
(MIP-1α)
standard curve range: 0.37-1,500 pg/mL
(IL-7)
standard curve range: 0.45-1,750 pg/mL
(TNFα)
standard curve range: 0.49-2,000 pg/mL
(IL-1β)
standard curve range: 0.49-2,000 pg/mL
(IL-12 (p70))
standard curve range: 0.49-2,000 pg/mL
(IL-2)
standard curve range: 0.49-2,000 pg/mL
(IL-5)
standard curve range: 0.61-2,500 pg/mL
(IFNγ)
standard curve range: 0.61-2,500 pg/mL
(MIP-3α)
standard curve range: 0.73-3,000 pg/mL
(IL-17A)
standard curve range: 0.92-3,750 pg/mL
(MIP-1β)
standard curve range: 1.22-5,000 pg/mL
(GM-CSF)
standard curve range: 1.46-6,000 pg/mL
(IL-10)
standard curve range: 1.46-6,000 pg/mL
(ITAC)
standard curve range: 1.83-7,500 pg/mL
(IL-4)
standard curve range: 18.3-75,000 pg/mL
(Fractalkine)
standard curve range: 7.93-32,500 pg/mL
(IL-23)
inter-assay cv: <15%
intra-assay cv: <10%

技术

multiplexing: suitable

检测方法

fluorometric (Luminex xMAP)

运输

wet ice

相关类别

一般描述

细胞因子是在适应性和先天免疫应答中起关键作用的免疫调节多肽。通用术语“细胞因子”包括肌肉因子（由肌肉细胞产生）、淋巴因子（由活化的Th细胞产生）、白介素（充当T细胞之间的介质）和趋化因子（负责T细胞迁移）。细胞因子是免疫系统的调节机制之一，作用于免疫应答的识别、激活或效应阶段，调节T细胞、B细胞和髓样细胞亚型的发育和功能活性。因此，涉及细胞因子的研究在更深入地了解免疫系统及其对大多数抗原的多方面反应（尤其是构成炎症过程的那些反应）方面起着重要作用。低水平的炎症涉及许多临床和亚临床疾病状态，例如自身免疫性疾病，心血管疾病，糖尿病，神经系统疾病和癌症。因此，测量细胞因子的微微克水平对于理解这些疾病的发病机理至关重要。

MILLIPLEX®人高灵敏度T细胞磁珠检测板用于同时定量人血浆、血清和细胞/组织培养上清液样品中以下分析物的任何或全部：趋化因子，GM-CSF，IFNγ，IL-1β，IL-2，IL-4，IL-5，IL-6，IL-7，IL-8，IL-10，IL-12（p70），IL-13，IL-17A，IL-21，IL-23，ITAC，MIP-1α，MIP-1，βMIP-3α
和TNFα。

Luminex® xMAP®平台使用磁珠免疫分析格式，以实现理想的速度和灵敏度，同时定量多种分析物，从而显著提高生产力，同时节省宝贵的样品量。

面板类型：细胞因子/趋化因子

特异性

交叉反应性
面板内无可检测的交叉反应性。
抗体与该面板中任何其他分析物之间的交叉反应性都为无法检测或可忽略。

应用

  • 分析物：趋化因子，GM-CSF，IFNγ，IL-1β，IL-2，IL-4，IL-5，IL-6，IL-7，IL-8，IL-10，IL-12（p70），IL-13，IL-17A，IL-21，IL-23，I-TAC，MIP-1α，MIP-1β，MIP-3α，TNF-α
  • 因为样品制备和试验设置有独特的功能，所以在运行试验之前请阅读整个方案。
  • 推荐的样品类型：血清、血浆或组织/细胞裂解物和培养上清液
  • 分析运行时间：过夜
  • 研究类别：炎症&免疫学
  • 研究子类别：免疫反应，代谢紊乱，慢性炎症性疾病，心血管疾病，神经科学

特点和优势

通过在此面板中选择可用的分析物来设计多重试剂盒。

联系

替代品：HSTCMAG-28SK；HSCYTMAG-60SK

储存及稳定性

试剂盒组件建议的存储温度为2-8°C。

其他说明

灵敏度：请参阅试剂盒方案了解单个检测试剂盒的灵敏度。
请联系技术服务部进行稀释度线性研究。

法律信息

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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警示用语：

Danger

危险分类

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

靶器官

Respiratory Tract

WGK

WGK 3

法规信息

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B Karshikoff et al.
Brain, behavior, and immunity, 57, 38-46 (2016-04-09)
Low-grade systemic inflammation has been implicated in chronic pain, as well as in comorbid diseases like depression and fatigue. We have previously shown that women's pain perception and regulation is more affected by systemic inflammation than that of men. Here
Bruna M Ascoli et al.
International journal of bipolar disorders, 7(1), 13-13 (2019-06-04)
Innate immune system dysfunction has been recognized as an important element in the pathophysiology of bipolar disorder (BD). We aimed to investigate whether there are differences in the response of macrophages derived from patients in the early stages and late
Malcolm Sanderson et al.
Sports (Basel, Switzerland), 8(12) (2020-12-05)
Swimming is a popular youth sport that is considered beneficial for cardiovascular fitness. However, the potential inflammatory outcomes of high intensity swimming in younger swimmers are unclear, as is the response of irisin, a myokine released during exercise with anti-inflammatory
Abstracts of the XXXVIII Congress of the Spanish Society of Physiological Sciences (SECF), 13-16 September 2016, Zaragoza, Spain.
Journal of physiology and biochemistry, 72 Suppl 1, 1-111 (2016-08-16)
Matteo Ravaioli et al.
Scientific reports, 10(1), 6063-6063 (2020-04-10)
With the aim to explore innovative tools for organ preservation, especially in marginal organs, we hereby describe a clinical trial of ex-vivo hypothermic oxygenated perfusion (HOPE) in the field of liver (LT) and kidney transplantation (KT) from Extended Criteria Donors
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Immunology Multiplex Assays | MILLIPLEX®

Immunology multiplex assays like MILLIPLEX® allow simultaneous investigation of immune biomarkers, saving time and resources in research.

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