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Key Documents

Safety Information

EZMCP1

Sigma-Aldrich

Human MCP-1 ELISA Kit

detects MCP-1 in 50 μL serum, plasma or cell culture supernatent

Synonym(s):

C-C motif chemokine 2, Monocyte chemoattractant protein 1

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About This Item

UNSPSC Code:
41116158
NACRES:
NA.84

Pricing and availability is not currently available.

Product Name

Human MCP-1 Conferma® ELISA, quantification of human MCP-1 in serum, plasma, or culture media samples.

clone

monoclonal

Quality Level

species reactivity

human

parameter

50 μL sample volume (4hr assay)

assay range

linearity: 94-106 (serum)%
linearity: 94-110 (plasma)%
inter-assay cv: <12.9%
intra-assay cv: <4.9%
standard curve range: 1.17-300 pg/mL
accuracy: 84-95%

technique(s)

ELISA: suitable

input

sample type plasma (K2 EDTA)
sample type serum
sample type cell culture supernatant

UniProt accession no.

application(s)

research use

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This Item
150357150356150268
separation technique

hydrophilic interaction (HILIC), supercritical fluid (SFC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

separation technique

hydrophilic interaction (HILIC), normal phase

particle size

5 μm

particle size

5 μm

particle size

5 μm

particle size

5 μm

matrix

fully porous particle

matrix

fully porous particle

matrix

fully porous particle

matrix

fully porous particle

product line

Purospher®

product line

Purospher®

product line

Purospher®

product line

Purospher®

pore size

120 Å pore size

pore size

120 Å pore size

pore size

120 Å pore size

pore size

120 Å pore size

General description

Human MCP-1 is a chemokine assoscited with the attraction of Monocytes and Basophils to the site of a pathology. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. In particilar this is a marker of inflammatory disorders. It binds to chemokine receptors CCR2 and CCR4.
This is a 96 well format sandwich ELISA which uses our Conferma® process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots.

Application

This Human MCP-1 Conferma® ELISA kit is used for the quantification of Human MCP-1 in serum, plasma, or culture media samples. One kit is sufficient to measure 37 unknown samples in duplicate.

Research Category:
  • Cytokines
  • Inflammation & Immunology
  • Immune Response
  • Metabolic Disease

Packaging

Two boxes; each to be analyzed separately.

Analysis Note

3 hour 45 minute assay, room temperature, 1:5 dilution recommended e.g.30 μL sample + 120μL of diluent (serum, plasma, or tissue culture medium)

Legal Information

CONFERMA is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

For research use only. Not for use in diagnostic procedures.

Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Regulatory Information

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Certificates of Analysis (COA)

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Articles

Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.

Protocols

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

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