EZIL6
Human IL-6 ELISA Kit
detect IL-6 in 50 μL serum, plasma or cell culture supernatent
Synonym(s):
B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, IL6, Interferon beta-2, Interleukin-6
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About This Item
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Product Name
Human IL-6 Conferma® ELISA, quantification of human IL-6 in serum, plasma, or culture media samples
Quality Level
species reactivity
human
parameter
50 μL sample volume (4hr assay)
assay range
linearity: 105-116%
(serum)
linearity: 88-105%
(plasma)
inter-assay cv: <15%
intra-assay cv: <5%
standard curve range: 1.17-300 pg/mL
accuracy: 91-109%
technique(s)
ELISA: suitable
input
sample type serum
sample type plasma (K2 EDTA)
sample type cell culture supernatant
UniProt accession no.
application(s)
research use
detection method
colorimetric (450nm/590nm)
Related Categories
1 of 4
This Item | H5147 | SAB4200797 | H1775 |
|---|---|---|---|
| species reactivity plant, bovine, rabbit, chicken, human, fish, insect, monkey, dog, rat, sea urchin, mouse | species reactivity bovine, chicken, guinea pig, plant, Drosophila, rat, human, nematode, rabbit, hamster | species reactivity chicken, rat, canine, human, monkey, mouse, bovine | species reactivity wheat germ, chicken, Sf9 cell line, rabbit, rat, human, mouse, Achlya |
| antibody form purified from hybridoma cell culture | antibody form ascites fluid | antibody form IgG fraction of antiserum | antibody form ascites fluid |
| clone BRM-22, monoclonal | clone BRM-22, monoclonal | clone polyclonal | clone AC-16, monoclonal |
| mol wt antigen ~70 kDa | mol wt antigen 70 kDa | mol wt ~70 kDa | mol wt antigen 88 kDa |
| isotype IgG1 | isotype IgG1 | isotype - | isotype IgG2b |
General description
This is a 96 well format sandwich ELISA which uses our Conferma® process to ensure a high degree of lot to lot consistency. The process involves the physiochemical characterization of the ELISAs critical reagents lot to lot, with finished kits being evaluated using a sample library to bridge between lots. For detailed information on the process and a Verification report for this assay please click hereConferma™ Sandwich ELISAs
Entrez Gene Summary:
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections.
UniProt Summary:
- Function: Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism.
- Size: 212 amino acids; 23,718 Da
- Subunit: Component of a hexamer of two molecules each of IL6, IL6R and IL6ST.
- Subcellular location: Extracellular region or secreted
- Disease: Rheumatoid arthritis systemic juvenile (RASJ). A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
- Similarity: Belongs to the IL-6 superfamily.
Application
Research Category:
- Cytokines
- Inflammation & Immunology
- Immune Response
- Metabolic Disease
Analysis Note
Legal Information
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recommended
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Learn about the Conferma® ELISA development and manufacturing methods that provide strong sample detection and long-term assay and lot consistency, giving you confidence in your research.
Protocols
This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.
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