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Merck
CN
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文件

CS0390

Sigma-Aldrich

线粒体染色试剂盒

1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)

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别名:
JC-1 dye
UNSPSC代码:
12352207
NACRES:
NA.32

质量水平

200

用途

 kit sufficient for 200 tests (of 1 mL cell suspensions)
 kit sufficient for 40 tests (of 5 mL cell suspensions)

包装

pkg of 1 kit

储存条件

dry at room temperature

技术

flow cytometry: suitable
protein staining: suitable

荧光

λex 490 nm; λem 530 nm (green) (JC-1 monomers)
λex 525 nm; λem 590 nm (red) (JC-1 aggregates)

应用

cell analysis
detection

检测方法

fluorometric

运输

wet ice

储存温度

2-8°C

相关类别

一般描述

在正常细胞中，JC-1染料能够聚集于线粒体基质中，形成红色的荧光聚集物。任何消耗线粒体跨膜电位的活动（如凋亡）都会影响线粒体中的JC-1染料聚集，导致这种染料分散到整个细胞中，从而使红色荧光（JC-1聚集体）转变为绿色荧光（JC-1单体）。本试剂盒可进行细胞染色，再通过荧光显微镜观察或流式细胞仪观测或测定荧光强度。

应用

线粒体电化学势梯度 (Δψ) 消耗被视为细胞凋亡的早期症状。本试剂盒采用阳离子亲脂性染料JC-1，可在活细胞中方便、快速地检测线粒体内膜电化学电位变化。

特点和优势

试剂盒提供：
  • 可简单、快速、方便地染色和分析线粒体完整性
  • 包含检测线粒体内膜电化学势变化所需的全部试剂
  • 包含抗生素缬氨霉素，可作为防止JC-1聚集的对照试剂
  • 可在绿色和红色通道中清晰地检测到荧光变化
  • 适用于贴壁细胞和悬浮细胞
  • 已经过Jurkat、U-937、HeLa和NIH3T3细胞的测试
  • 可通过荧光显微镜或流式细胞仪观测和分析荧光信号

仅试剂盒组分

产品编号
说明
  • DMSO 1 mL
  • JC Staining Buffer 5X 120 mL
  • JC-1 1 mg
  • Valinomycin Ready Made .1 mL

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Robert L Elliott et al.
PloS one, 14(4), e0214586-e0214586 (2019-04-02)
Many cells are cultured in media that contains an antibiotic to prevent bacterial contamination. Mycoplasma and other bacterial contamination is a serious problem for those involved in cell culture. Antibiotics in the media helps prevent this contamination and make life
Maria C Zanellati et al.
Frontiers in genetics, 6, 78-78 (2015-03-31)
Mutations in PARK2, encoding Parkin, cause an autosomal recessive form of juvenile Parkinson Disease (JPD). The aim of the present study was to investigate the impact of PARK2 mutations on mitochondrial function and morphology in human skin fibroblasts. We analyzed
Whasun Lim et al.
Biology of reproduction, 95(4), 82-82 (2016-09-02)
Luteolin is a natural compound known for its anticancer effects on various human cancers by regulating signal transduction cascades. However, the effects of luteolin on human placental choriocarcinoma are not known. Results of the present study revealed that luteolin decreased
A Cossarizza et al.
Biochemical and biophysical research communications, 197(1), 40-45 (1993-11-30)
A new method for the cytofluorimetric analysis of mitochondrial membrane potential in intact cells has been developed by using the lipophilic cationic probe 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1), whose monomer emits at 527 nm after excitation at 490 nm. Depending on the
Sunwoo Park et al.
Molecular human reproduction, 23(12), 842-854 (2017-11-10)
Does the flavonoid naringenin inhibit proliferation of human endometriosis cells? Naringenin suppresses proliferation and increases apoptosis via depolarization of mitochondrial membrane potential and generation of reactive oxygen species (ROS) in human endometriosis cells. For management of endometriosis, hormonal therapy is
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商品

Mitochondrial Stress and ROS

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

线粒体应激和ROS

氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。

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