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Merck
CN

T8935

Sigma-Aldrich

BlueView Nucleic Acid Stain

in 10× TAE buffer, powder blend

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MDL编号:
UNSPSC代码:
12161700

等级

for molecular biology

形式

powder blend

检测灵敏度测量范围

<250 ng/band

储存温度

room temp

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应用

BlueView is a quick, safe alternative to ethidium bromide staining for nucleic acid electrophoresis. BlueView can be used directly as the running buffer and in the gel for instant staining of the bands of nucleic acids during electrophoresis, visible in ambient light. Bands can be excised from the gel without the use of UV which can cause nicking. BlueView stained bands can be used for PCR, ligation, labeling, restriction digestion, and Southern blotting.

重悬

Reconstitution with water produces a 10× stock solution of the BlueView stain in 10× TAE or TBE buffer. The bottles are large enough to contain the concentrate. Dilute the 10× stock solution to the desired concentration before use. Concentrations of TBE and TAE between 0.5 and 1× are commonly used for nucleic acid electrophoresis. A 20-fold dilution of the stock solution is recommended for low background staining and optimum signal to noise.

法律信息

BlueView is a trademark of Sigma-Aldrich Co. LLC

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

法规信息

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Thorunn Rafnar et al.
Nature genetics, 43(11), 1104-1107 (2011-10-04)
Ovarian cancer causes more deaths than any other gynecologic malignancy in developed countries. Sixteen million sequence variants, identified through whole-genome sequencing of 457 Icelanders, were imputed to 41,675 Icelanders genotyped using SNP chips, as well as to their relatives. Sequence

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