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一般描述
TrypZean®溶液，1×是通过玉米中表达的重组牛胰蛋白酶TrypZean配制，并利用ProdiGene′专有的转基因植物蛋白表面系统生成。该产物经优化处理，适用于无血清和补充血清的贴壁细胞培养基中的细胞解离。
应用
TrypZean®溶液，1×已用于：
- 在DNA提取过程中悬浮颗粒
- 将细胞消化为单细胞悬液，用于2D细胞的药物分型
- 重组胰蛋白酶，诱导IPT-AF培养基中继代培养的Vero细胞分裂
特点和优势
TrypZean®溶液具有以下特点和优势：
- 不含动物组分-消除病毒、BSE或其他潜在外来因子的风险
- TrypZean是重组胰蛋白酶-使用相同的酶（具有相同的动力学）进行细胞分离意味着最少的实验方案更改
- 酶抑制-大豆胰蛋白酶铁罐剂及其他抑制剂对TrypZean的作用与对天然胰蛋白酶的作用相同（以重量计）
- 高纯度-TrypZean提供更高的特异性，消除低纯度酶中出现的污染活性
- 方便快捷-TrypZean溶液是一种无菌过滤溶液，以最佳浓度配制而民，旨在解离贴壁细胞系
法律信息
Trypzean is a registered trademark of ProdiGene, Inc.
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Samia Rourou et al.
Biotechnology progress, 25(6), 1752-1761 (2009-09-22)
This work describes the development of an animal-component free medium (IPT-AFM) that allows an optimal growth of Vero cells, an adherent cell line used for the production of viral vaccines. Statistical experimental design was applied to identify crucial nutrients that
Maria T Nelson et al.
Cell reports, 26(8), 2227-2240 (2019-02-21)
Metagenomic sequencing is a promising approach for identifying and characterizing organisms and their functional characteristics in complex, polymicrobial infections, such as airway infections in people with cystic fibrosis. These analyses are often hampered, however, by overwhelming quantities of human DNA, yielding
Katja Peschke et al.
EMBO molecular medicine, 14(4), e14876-e14876 (2022-02-05)
Despite the advance and success of precision oncology in gastrointestinal cancers, the frequency of molecular-informed therapy decisions in pancreatic ductal adenocarcinoma (PDAC) is currently neglectable. We present a longitudinal precision oncology platform based on functional model systems, including patient-derived organoids
Maria T Nelson et al.
Cell reports, 26(8), 2227-2240 (2019-02-21)
Metagenomic sequencing is a promising approach for identifying and characterizing organisms and their functional characteristics in complex, polymicrobial infections, such as airway infections in people with cystic fibrosis. These analyses are often hampered, however, by overwhelming quantities of human DNA, yielding
Reinforcement Learning Recruits Somata and Apical Dendrites across Layers of Primary Sensory Cortex.
Clay O Lacefield et al.
Cell reports, 26(8), 2000-2008 (2019-02-21)
The mammalian brain can form associations between behaviorally relevant stimuli in an animal's environment. While such learning is thought to primarily involve high-order association cortex, even primary sensory areas receive long-range connections carrying information that could contribute to high-level representations.
商品
Millicell® hanging cell culture inserts for staining and microscopic analysis directly from the insert. Learn to utilize hanging cell culture inserts in barrier measurements.
实验方案
实体瘤以三维(3D)空间构象生长，导致氧和营养物质以及其他物理和化学应力的异质性暴露。为了模拟三维空间构象，在肿瘤研究中普遍使用三 维体外培养模型。
Step-by-step tumorpshere formation protocol that can used to generate 3D cancer stem cell spheroids.
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
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