推荐产品
一般描述
面向全基因组扩增（WGA）的 SeqPlex™ Enhanced DNA 扩增试剂盒专用于对极小量或降解/高度片段化的 DNA 进行下一代测序（NGS）。 染色质免疫沉淀（ChIP）或福尔马林固定的石蜡包埋组织样品（FFPE）的DNA含量往往无法满足成功制备下一代测序文库的需求。通过 SeqPlex 试剂盒可对这类 DNA 以及其他用于 NGS 的小量/高度片段化 DNA 样品进行预扩增。 该试剂盒是 WGA 产品线的延伸，旨在集成到 Illumina®、SOLiD™ 或 454 测序工作流程中。
应用
SeqPlex™ DNA 扩增试剂盒已用于全基因组扩增。
特点和优势
- 采用随机引物技术扩增片段化DNA，如ChIP或FFPE
- 有助于对低至100 pg的ChIP DNA进行测序
- 采用增强型引物，可获得完整的基因组覆盖、最低的序列偏差、引物清除以及适用于二代测序的扩增子大小
- 可兼容用于二代测序的Illumina®、SOLiD™或454文库制备
其他说明
SEQXE-500RXN 是按需生产。获取更多信息，请发邮件至techserv@sial.com 联系技术服务。
法律信息
Illumina is a registered trademark of Illumina, Inc.
SOLiD is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
试剂盒组分也可单独购买
产品编号
说明
化学品安全说明书
- W4502Water, Nuclease-Free Water, for Molecular Biology化学品安全说明书
- Library Preparation Buffer
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Dimiter Kunnev et al.
Journal of biological methods, 2(4) (2015-01-01)
Nascent strand capture and release (NSCR) is a method for isolation of short nascent strands to identify origins of DNA replication. The protocol provided involves isolation of total DNA, denaturation, size fractionation on a sucrose gradient, 5'-biotinylation of the appropriate
Takuya Hayakawa et al.
Cytogenetic and genome research, 161(8-9), 437-444 (2021-11-25)
E/L Repli-seq is a powerful tool for detecting cell type-specific replication landscapes in mammalian cells, but its potential to monitor DNA replication under replication stress awaits better understanding. Here, we used E/L Repli-seq to examine the temporal order of DNA
Joshua A Udall et al.
Frontiers in plant science, 10, 1541-1541 (2019-12-13)
One of the extraordinary aspects of plant genome evolution is variation in chromosome number, particularly that among closely related species. This is exemplified by the cotton genus (Gossypium) and its relatives, where most species and genera have a base chromosome
SF3B1 mutant MDS-initiating cells may arise from the haematopoietic stem cell compartment.
Mian S A, et al.
Nature Communications, 6, 10004-10004 (2015)
Xiang Yu et al.
Genome research, 27(7), 1238-1249 (2017-04-08)
Type II topoisomerases orchestrate proper DNA topology, and they are the targets of anti-cancer drugs that cause treatment-related leukemias with balanced translocations. Here, we develop a high-throughput sequencing technology to define TOP2 cleavage sites at single-base precision, and use the
实验方案
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。
Sigma Imprint染色质免疫沉淀试剂盒利用检测板系统，可实现快速的高通量ChIP检测
The Sigma Imprint Chromatin Immunoprecipitation Kit uses a plate based system to allow rapid ChIP assays in a high throughput format
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