product name
碳酸氢钠 溶液, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture
无菌性
sterile-filtered
质量水平
产品线
BioReagent
形式
solution (7.5%)
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
pH值（酸碱度）
7.8-8.2
储存温度
2-8°C
SMILES字符串
[Na+].OC([O-])=O
InChI
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
InChI key
UIIMBOGNXHQVGW-UHFFFAOYSA-M
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一般描述
碳酸氢钠(NaHCo3)是实验室常用的碱性缓冲剂(5)。加入细胞培养基后，配合培养箱中的二氧化碳共同维持适宜的pH。除了缓冲作用，碳酸氢钠还能作为钠源，在发酵时用于合成葡糖酸钠。
应用
碳酸氢钠溶液可用于：
- 作为Hanks′平衡盐溶液(HBSS)和recording medium组分，用于大鼠或小鼠组织外植体的培养
- 作为Dulbecco′改良Eagle′培养基(DMEM)组分，用于小鼠组织外植体的培养
- 作为最低必需培养基(MEM)组分，用于研究对人结直肠腺癌细胞和乳腺腺癌的细胞毒性
- 制备胶原混合物液滴，用于3D胶原出芽实验
- 制备胶原基质，用于大鼠表皮角质形成细胞(REK)器官型培养
制备说明
用细胞培养级水配制。
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Phenotypic Modulation of Adipose-Derived Stem Cells and Fibroblasts Treated with Povidone--Iodine and Chlorhexidine in Mono and Coculture Models
Chelmu? BA, et al.
Biomedicines, 11(7), 1855-1855 (2023)
Nicoline Dorothea Jakobsen et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 178, 106282-106282 (2022-08-23)
Rat epidermal keratinocyte (REK) Organotypic culture (ROC) is an epidermis model that is robust and inexpensive to develop and maintain, and it has in previous studies been shown to have permeability characteristics close to those of human skin. Here, we
Mette Flinck et al.
Cell cycle (Georgetown, Tex.), 17(9), 1056-1067 (2018-06-14)
Precise acid-base homeostasis is essential for maintaining normal cell proliferation and growth. Conversely, dysregulated acid-base homeostasis, with increased acid extrusion and marked extracellular acidification, is an enabling feature of solid tumors, yet the mechanisms through which intra- and extracellular pH
Anna E Engler et al.
Cell reports, 33(13), 108553-108553 (2020-12-31)
There is an increasing appreciation for the heterogeneity of myeloid lineages in the lung, but relatively little is known about populations specifically associated with the conducting airways. We use single-cell RNA sequencing, flow cytometry, and immunofluorescence to characterize myeloid cells
Caroline Volz et al.
Cell reports, 31(4), 107568-107568 (2020-04-30)
Anti-angiogenic treatment targeting vascular endothelial growth factor (VEGF)-VEGFR2 signaling has shown limited efficacy in lung cancer patients. Here, we demonstrate that inhibition of VEGFR2 in tumor cells, expressed in ∼20% of non-squamous non-small cell lung cancer (NSCLC) patients, leads to
实验方案
3-D Culture Matrix™胶原I可单独或与其他基底膜成分一起用作在其上生长细胞的凝胶或培养基添加剂，用于研究体外三维的细胞生长和分化。
3-D Culture Matrix™ Collagen I facilitates cellular growth and differentiation in vitro, offering versatile applications in biomedical research.
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