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Merck
CN

S6402

Sigma-Aldrich

链霉亲和素 −Cy3 来源于阿维丁链霉菌

buffered aqueous solution

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MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.46

偶联物

CY3 conjugate

质量水平

形式

buffered aqueous solution

标记范围

3-9 mol fluorochrome per mol protein

技术

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100

运输

wet ice

储存温度

2-8°C

一般描述

链霉亲和素是一种同源四聚体蛋白。一分子链亲和素通过非共价相互作用与四分子生物素结合——这种相互作用基本上是不可逆的。链霉亲和素对生物素具有高亲和力,广泛用作标记工具及涉及结合蛋白的研究。

应用

阿维迪尼链霉菌链霉亲和素 −Cy3 已用于
  • :双重免疫荧光染色检测生物素凝集素
  • 免疫荧光分析中的胰岛素和 C 肽染色
  • 双重免疫荧光,以验证是否存在充满生物胞素的细胞
  • 与生物素化抗体联合应用于免疫组化和免疫细胞化学
  • 由于光谱重叠,与传统的罗丹明滤光片联用

特点和优势

Cy3 染料的光谱特性:
  • 吸收最大值 552nm
  • 发射最大值 565nm
  • 摩尔消光系数 1.3 x 10-5 M-1cm-1

外形

0.01 M 磷酸盐缓冲液 (pH 7.4) 中的溶液,含 1% 牛血清白蛋白和 15 mM 叠氮化钠

法律信息

Cy3 is a trademark of Cytiva

免责声明

除非我们的目录或产品随附的其他公司文件中另有说明,否则我们的产品预期仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、 体外 诊断用途、 离体 或 体内 治疗用途或任何类型的消费或人类或动物应用。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

常规特殊物品
含少量动物源组分生物产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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Hiroyuki Nobusue et al.
Cell and tissue research, 332(3), 435-446 (2008-04-04)
We established a preadipocyte cell line from mature adipocytes obtained from subcutaneous fat tissue of green fluorescent protein (GFP) transgenic mice. The floating top layer, containing mature adipocytes, was isolated from subcutaneous fat tissue by collagenase digestion and filtration. Fluorescence-activated
Ji-Myung Bae et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 33(6), 1126-1140 (2018-02-07)
The Sp7/Osterix transcription factor is essential for bone development. Mutations of the Sp7 gene in humans are associated with craniofacial anomalies and osteogenesis imperfecta. However, the role of Sp7 in embryonic tooth development remains unknown. Here we identified the functional
Agnieszka E Gorska et al.
The American journal of pathology, 163(4), 1539-1549 (2003-09-26)
We have previously shown that expression of a dominant-negative type II transforming growth factor-beta receptor (DNIIR) in mammary epithelium under control of the MMTV promoter/enhancer causes alveolar hyperplasia and differentiation in virgin mice. Here we show that MMTV-DNIIR female mice
Niklas Schwarz et al.
eLife, 8 (2019-09-10)
Most of our knowledge on human CNS circuitry and related disorders originates from model organisms. How well such data translate to the human CNS remains largely to be determined. Human brain slice cultures derived from neurosurgical resections may offer novel
Miroslav Hons et al.
Nature immunology, 19(6), 606-616 (2018-05-20)
Although much is known about the physiological framework of T cell motility, and numerous rate-limiting molecules have been identified through loss-of-function approaches, an integrated functional concept of T cell motility is lacking. Here, we used in vivo precision morphometry together

商品

可用的荧光原位杂交(FISH)过程、试剂和设备。

Available Fluorescent in situ hybridization (FISH) procedures, reagents and equipment.

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