P9279
磷脂酶 A 2 from honey bee venom (Apis mellifera)
salt-free, lyophilized powder, 600-2400 units/mg protein
别名:
卵磷脂酶 A, 磷脂酰胆碱 2-酰基水解酶, 聚乳酸 2
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-637-7
MDL number:
Specific activity:
600-2400 units/mg protein
form
salt-free, lyophilized powder
specific activity
600-2400 units/mg protein
mol wt
14.5 kDa
UniProt accession no.
storage temp.
−20°C
Quality Level
Gene Information
honey bee ... Pla2(406141)
General description
蜜蜂毒液（西方蜜蜂）来源磷脂酶A2是钙依赖性脂肪分解酶。
Application
蜂毒磷脂酶 A2（意大利蜜蜂）已用于
- 确定氧合和缺氧-再氧合过程中近端肾小管节段 (PTS) 的活力
- 在治疗 集胞藻的光系统 II (PSII) 二聚体复合物 研究析氧活性的变化
- 作为磷脂酶活性测定的标准品
Biochem/physiol Actions
水解 β-两性甘油磷脂的酯键。首选底物为磷脂酰胆碱、磷脂酰乙醇胺及其缩醛磷脂类似物。磷脂酰肌醇和磷脂酰丝氨酸也被水解。其会攻击完整细胞膜上的磷脂。
Analysis Note
双缩脲法测定蛋白质。
Other Notes
一个单位将在 pH8.9，25°C 下每分钟将 1.0μmole 大豆L-α-磷脂酰胆碱水解成 L-α-溶血磷脂酰胆碱和脂肪酸。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
Milleporin-1, a new phospholipase A2 active protein from the fire coral Millepora platyphylla nematocysts.
Radwan FF and Aboul-Dahab HM
Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP, 139(4), 267-272 (2004)
Lipids in oxygen-evolving photosystem II complexes of cyanobacteria and higher plants.
Sakurai I
Journal of Biochemistry, 140(2), 201-209 (2006)
Third calcium ion found in an inhibitor-bound phospholipase A2.
Sekar K
Acta Crystallographica Section D, Biological Crystallography, 62, 392-397 (2006)
Phospholipase A2 activity can protect renal tubules from oxygen deprivation injury.
Zager RA
Proceedings of the National Academy of Sciences of the USA, 90(17), 8297-8301 (1993)
P K Larsson et al.
The Journal of biological chemistry, 273(1), 207-214 (1998-02-07)
Recently, the cloning of a novel Ca2+-independent phospholipase A2 (iPLA2) from Chinese hamster ovary cells as well as from mouse and rat sources containing a C-terminal lipase motif and eight N-terminal ankyrin repeats has been described. In this report we
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