P8804
磷脂酶 C，磷脂酰肌醇特异性 来源于蜡样芽胞杆菌
buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)
别名:
1-磷脂酰- D - 肌醇肌醇磷酸水解酶，环磷酸盐形成, PI-PLC
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-638-2
MDL number:
Specific activity:
≥1,000 units/mg protein (Lowry)
Biological source:
Bacillus sp. (Bacillus cereus)
biological source
Bacillus sp. (Bacillus cereus)
Quality Level
form
buffered aqueous glycerol solution
specific activity
≥1,000 units/mg protein (Lowry)
mol wt
28 kDa
foreign activity
Phopholipase C (lecithinase) ≤1 units/mg protein, Sphingomyelinase ≤40 units/mg protein
storage temp.
2-8°C
Gene Information
Bacillus cereus E33L ... BCZK3513(3026815)
General description
来自蜡状芽孢杆菌的磷脂酶 C（PI-PLC）具有不规则的三糖磷酸异构酶（TIM）-桶结构，具有八个标准的平行 β 桶。它是一种 28 kDa 的蛋白质。
Application
蜡状芽孢杆菌特有的磷脂酶 C（磷脂酰肌醇）可用于：
- 裂解底物对硝基苯基磷酰胆碱（p-NPPC）和对硝基苯基磷酰磷酸（p-NPP）
- 裂解 lynx1 蛋白的糖基磷脂酰肌醇（GPI）锚定及其与质膜的分离
- 裂解免疫标记的 HeLa 细胞
Biochem/physiol Actions
磷脂酶 C（PI-PLC）通过裂解糖基磷脂酰肌醇释放二酰基甘油。它具有广泛的底物特异性，其活性受金属离子和表面活性剂的影响。
用于释放锚定的 GPI 膜中的蛋白质。
Physical form
含 10 mM Tris-HCl，pH 8.0 和 10 mM EDTA 的 60％（v/v）甘油溶液
Analysis Note
根据 Ellman 等人 的研究测定乙酰胆碱酯酶。
Other Notes
在 30°C、pH 7.4 条件下，每分钟释放 1 个单位的乙酰胆碱酯酶。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
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商品
Glycosylphosphatidylinisotol- (GPI) anchored proteins have been identified throughout a broad range of eukaryotic species ranging from humans to insects, yeasts, bacteria, and fungi, suggesting that they are an ancient modification.
GPI锚定糖蛋白
相关内容
Lynx1 shifts alpha4beta2 nicotinic receptor subunit stoichiometry by affecting assembly in the endoplasmic reticulum
Nichols WA, et al.
The Journal of Biological Chemistry, 289(45), 31423-31432 (2014)
High-level expression of recombinant phospholipase C from Bacillus cereus in Pichia pastoris and its characterization
Seo KH and Rhee JI
Biotechnology Letters, 26(19), 1475-1479 (2004)
Crystal structure of phosphatidylinositol-specific phospholipase C from Bacillus cereus in complex with glucosaminyl (alpha1? 6)-d-myo-inositol, an essential fragment of GPI anchors
Heinz DW, et al.
Biochemistry, 35(29), 9496-9504 (1996)
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| P8804-25UN | 04061832856858 |
| P8804-5UN | 04061832856865 |