推荐产品
产品线
BioXtra
质量水平
方案
≥99% (titration)
表单
powder
杂质
Insoluble matter, passes filter test
灼烧残渣 (900 °C)
≤0.5% (as SO4)
缺失
≤0.5% loss on drying, 110°C
有效pH范围
6.1-7.5
pKa (25 °C)
6.8
mp
>300 °C (lit.)
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相关类别
一般描述
PIPES是乙磺酸缓冲盐系列之一，由Good等人首先开发 以满足某些标准：中值pKa，最大水溶性和在所有其他溶剂中的最小溶解度，最小盐效应，pKa随温度的变化最小，化学和酶学稳定，在可见光或紫外光谱范围的最小吸收，易于合成。由于它在37 °C的pKa接近生理pH，PIPES还可用于细胞培养工作。
应用
使用PIPES用于分离琼脂糖凝胶中的乙醛酸化RNA、核酸酶S1的RNA作图以及核糖核酸酶保护检测的实验方法已被报道。PIPES已被用作戊二醛固定组织样品的缓冲液。,
PIPES已被用于蛋白结晶。， PIPES在tubulin
α和β亚基在免疫吸附凝胶上解析后进行重构的应用已被描述。PIPES已被推荐用于半胱天冬酶3、6、7和8体外研究的缓冲液中。
一项已发表的研究表明了PIPES在蛋白质分析等应用中可作为非金属离子络合缓冲液。PIPES在细胞培养中已被用于如在大肠杆菌中对热稳定突变膜蛋白进行工程化改造等应用。
PIPES已被用于蛋白结晶。， PIPES在tubulin
α和β亚基在免疫吸附凝胶上解析后进行重构的应用已被描述。PIPES已被推荐用于半胱天冬酶3、6、7和8体外研究的缓冲液中。
一项已发表的研究表明了PIPES在蛋白质分析等应用中可作为非金属离子络合缓冲液。PIPES在细胞培养中已被用于如在大肠杆菌中对热稳定突变膜蛋白进行工程化改造等应用。
联系
Sigma-Aldrich公司提供生物性能认证的细胞培养用PIPES(产品编号 P1851) 以及其他几种不同的盐类，以为缓冲液制备提供便利。
制备说明
缓冲液制备可通过向PIPES游离酸中加入碱溶液，滴定到适当的pH值，或采用等摩尔的钠盐溶液和二钠盐溶液混合滴定到适当的pH值。
分析说明
Trace elemental analyses have been performed on the BioXtra PIPES; Certificate of Analysis provides lot-specific results. BioXtra PIPES is for applications which require tight control of elemental content.
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
The use of Hanks'--pipes buffers in the preparation of human, normal leucocytes for TEM observation.
S Haviernick et al.
Journal of microscopy, 135(Pt 1), 83-88 (1984-07-01)
It is suggested that the use of Hanks' + pipes + sucrose buffers, in combination with glutaraldehyde and osmium tetroxide fixatives, represent an excellent mode of preparation of fresh and cultured peripheral blood leucocytes, not only for transmission electron microscopy
K E Loesser et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 34(11), 1477-1485 (1986-11-01)
We have modified the Lowicryl K4M low-temperature dehydration and embedding procedure for immunoelectron microscopy to provide improved ultrastructural detail and facilitate the localization of actin and tubulin in isolated rat adrenocortical cells, chick spinal cord with attached dorsal root ganglia
Sambrook , J. and Russell, D.W.
Molecular Cloning: A Laboratory Manual, 7-7 (2001)
S A Moore et al.
Journal of molecular biology, 312(3), 511-523 (2001-09-21)
Human bile salt-stimulated lipase (BSSL), which is secreted from the pancreas into the digestive tract and from the lactating mammary gland into human milk, is important for the effective absorption of dietary lipids. The dependence of BSSL on bile acids
A Giraudel et al.
Biochemistry, 37(24), 8724-8734 (1998-06-24)
The dissociation and separation of the tubulin alpha- and beta-subunits have been achieved by binding alpha-subunits to an immunoadsorbent gel and selectively inducing release of free beta-subunits. The immunoadsorbent gel was prepared by coupling the monoclonal antibody YL1/2 to Sepharose
实验方案
细胞染色可分为四个步骤：细胞制备、固定、抗体孵育和评估。
Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.
TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer
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