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Merck
CN

P5899

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt ≥300,000, powder, γ-irradiated, suitable for cell culture, BioXtra

别名:

PDL HBr

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关于此项目

化学文摘社编号:
25988-63-0
UNSPSC Code:
12352202
eCl@ss:
32160406
PubChem Substance ID:
24898718
NACRES:
NA.75
MDL number:
MFCD00237230

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产品名称

聚-L-赖氨酸 氢溴酸盐, mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

biological source

synthetic (organic)

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

mol wt

≥300,000

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm2

impurities

<10% water (by Karl Fischer)

solubility

H2O: soluble 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow

shipped in

ambient

storage temp.

−20°C

Quality Level

200

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Analysis Note

分子量基于粘度。同样通过 MALLS 分析。

Application

多聚-L-赖氨酸氢溴酸盐已用于包被:
  • 在一项虹鳟鱼肌小管形成的研究中在接种肌肉细胞前包被2孔培养板
  • 用于体外神经元干细胞分化的96孔板
  • 用于原代星形胶质细胞培养物的T75 瓶

Biochem/physiol Actions

多聚-L-赖氨酸可用作涂层物质，以确保细胞在培养物表面更好地附着。研究表明多聚-L-赖氨酸(PLL)包被的培养板中细胞生长速度加快。在包被PLL的底物上培养间充质干细胞可维持这些细胞的干性特征（stemness properties）并抑制衰老。包被PLL的培养板表现出改善的MSC生长，并使与细胞粘附、分化、增殖和信号传导相关的基因表达上调。此外，包被PLL的多孔表面改善细胞增殖和MSC的成骨分化。

Disclaimer

无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。

Other Notes

有关聚氨基酸的其他技术信息，请访问 聚氨基酸常见问题解答资源。
聚-L-赖氨酸是一种带正电荷的氨基酸聚合物，每个赖氨酸残基约有一个HBr。 氢溴酸盐可使聚-L-赖氨酸呈可溶于水的结晶形式。 在β结构中可能会发现少量的产物，因为HBr干扰氨基与羧基或含N或O部分之间的氢键。

存储类别

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000315801
SLCL5836
0000315801
SLCL5836
SLCF0030

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Direct addition of poly-lysine or poly-ethylenimine to the medium: A simple alternative to plate pre-coating
Alexander Faussner, et al.
PLoS ONE, 17(7) (2022)
Sonja Stadler et al.
BMC cell biology, 5(1), 44-44 (2004-11-24)
Between cell divisions the chromatin fiber of each chromosome is restricted to a subvolume of the interphase cell nucleus called chromosome territory. The internal organization of these chromosome territories is still largely unknown. We compared the large-scale chromatin structure of
Gustavo Castro et al.
ISRN ophthalmology, 2013, 752161-752161 (2014-02-22)
Purpose. The purpose of this work was to evaluate the retinal integration and differentiation of neurospheres formed by stem cells and mouse neural progenitor cells injected intravitreally in mice eyes with retinal injury. Methods. Eight male C57BL mice, 8 weeks
Polylysine for skin regeneration: A review of recent advances and future perspectives
Payam Zarrintaj, et al.
Journal of Separation Science, 5(7) (2021)
Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo
June Seok Heo, et al.
BioMed Research International (2016)
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商品

Extracellular Matrix Proteins and Tools for Cell Culture Optimization

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Poly-lysine Types for Biomedical Applications

Poly-Lysine enhances cell binding with positively-charged surface ions, optimizing electrostatic interaction on culture surfaces for increased cell attachment.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

细胞外基质蛋白质和细胞培养优化工具

细胞外基质蛋白质，如层粘连蛋白、胶原蛋白和纤连蛋白，可用作细胞培养中的细胞附着基底。

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实验方案

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

多聚-L-赖氨酸细胞附着实验方案

使用多聚-赖氨酸将细胞粘附在固相基质上，多聚-赖氨酸可增强细胞膜表面的负电荷离子与培养表面之间的静电相互作用。

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