product name
磷酸酶抑制剂混合物2, aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
形式
aqueous solution (dark coloration may develop upon storage, which does not affect the activity)
质量水平
运输
wet ice
储存温度
2-8°C
一般描述
磷酸酶 抑制剂混合液2是一种成分混合物,旨在抑制 修饰细胞提取物中蛋白质的酶,从而提高完整蛋白质 产量。它已经过优化和测试,适用于各种动物 组织和细胞质提取物。
应用
磷酸酶抑制剂混合物可通过向能够对细胞提取物中存在的蛋白进行修饰的酶添加抑制剂来提高完整蛋白的产量。 该产品已针对酪氨酸蛋白磷酸酶、酸性和碱性磷酸酶进行了优化和测试。 它已对来自包括牛肝和人胎盘的动物组织的细胞提取物和兔肌肉细胞提取物进行了测试。
生化/生理作用
该混合物含有具有特异性抑制属性的独立成分。 原钒酸钠可抑制多种ATP酶、蛋白酪氨酸磷酸酶和其他磷酸转移酶。 钼酸钠可抑制酸性和磷蛋白磷酸酶。 酒石酸钠可抑制酸性磷酸酶。 咪唑可抑制碱性磷酸酶。
特点和优势
该化合物是激酶磷酸酶生物学研究的特色产品。点击此处以发现更多的激酶磷酸酶生物学研究特色产品。可访问sigma.com/discover-bsm了解更多关于用于其他研究领域生物活性小分子。
注意
该产品在供货后可在推荐的2-8°C储存温度下稳定两年。
制备说明
该混合物以透明溶液形式提供。 它已通过无菌填充的瓶子中的0.2 μm膜进行了无菌过滤。 一毫升将可抑制来自人胎盘、牛肝、兔肌肉、A431或Jurkat细胞提取物的100,000 x g上清液中发现的蛋白浓度约为5 mg/mL的磷酸酶活性。
相关产品
产品编号
说明
价格
通常也和此产品一起购买
警示用语:
Danger
危险声明
危险分类
Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
WGK
WGK 2
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磷酸酶抑制剂对于所有类型的磷酸化研究都至关重要。对比磷酸酶抑制剂混合物,选择更适合您的研究的产品。
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