应用
磷酸酶抑制剂混合物可通过向能够对细胞提取物中存在的蛋白进行修饰的酶添加抑制剂来提高完整蛋白的产量。 该产品已经在来源于包括人胎盘和牛肝的动物组织以及A431、CHO和U937细胞的提取物上进行了测试。它已经优化并测试用于通过pNPP进行测量的碱性磷酸酶的L-同工酶以及丝氨酸-苏氨酸蛋白磷酸酶。
生化/生理作用
该混合物含有具有特异性抑制属性的独立成分。 斑蝥素可抑制蛋白磷酸酶2A。 (-)-对草酸溴左旋咪唑可抑制碱性磷酸酶的L-同工酶。 花萼海棉诱癌素A可抑制蛋白磷酸酶1和2A。
注意
该产品在供货后可在推荐的2-8°C储存温度下稳定四年。
制备说明
该混合物以溶于二甲基亚砜的透明溶液形式提供。推荐按每500 mg来自组织或细胞提取的蛋白采用1 mL的混合物对其稀释。 通常使用1%（v/v）的终浓度，其可抑制存在于人胎盘和牛肝27,000 × g上清液以及A431、CHO和U937提取物100,000 × g上清液中的磷酸酶活性。
WGK
WGK 1
闪点(°F)
188.6 °F
闪点(°C)
87 °C
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磷酸酶抑制剂对于所有类型的磷酸化研究都至关重要。对比磷酸酶抑制剂混合物，选择更适合您的研究的产品。
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