推荐产品
技术
cDNA synthesis: suitable
运输
wet ice
储存温度
−20°C
一般描述
MystiCq ®microRNA cDNA Synthesis Mix 试剂盒从总 RNA 或预富集样品或 microRNA 开始，包含将成熟 microRNA 转化为 qPCR 的 cDNA 模板所需的所有成分。 由于 microRNA 不是天然进行多聚腺苷酸化，因此 cDNA 合成过程的第一步是通过 poly(A) 聚合酶反应使 microRNA多聚腺苷酸化。 接下来，添加 ReadyScript 逆转录酶、oligo-dT 接头引物和其它必要试剂，将多聚腺苷酸化的 microRNA 转化为 cDNA。 接头引物的5' 端包含一个与 MystiCq 通用 PCR 引物的序列互补的独特序列，该序列是MystiCq microRNA qPCR 检测系统的另一个成分。该试剂盒还包含一个针对 SNORD44 的特异性阳性对照引物， SNORD44 是一种在大多数人体组织中广泛表达的小核仁 RNA。 试剂盒中包括足量的Poly(A)拖尾缓冲液和 MicroRNA cDNA 反应混合物，以允许不使用 poly(A) 聚合酶和不使用逆转录酶的对照反应。为了分析单个 microRNA，将 MystiCq 通用 PCR 引物（靶向接头引物序列）与 MystiCq® microRNA qPCR Assay 引物和 MystiCq microRNA SYBR ®Green qPCR ReadyMix ™一起使用。
应用
MystiCq® 微小 RNA cDNA 合成混合物已用于通过逆转录 miRNA 第一链 cDNA 合成，用于从总 RNA 开始的定量聚合酶链式反应（qPCR）
特点和优势
- 如果需要，单独的多腺苷酸化和逆转录步骤允许优化方案的可能性
- 生成的 cDNA 可存档备用
- 单一 RT 反应可提供多达 1000 个 qPCR 反应
- 包括用于大多数人体组织的阳性对照引物
包装
默认反应体积为 20μL
法律信息
MystiCq is a registered trademark of QIAGEN Beverly Inc.
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
仅试剂盒组分
产品编号
说明
- Poly (A) Tailing Buffer (5X)
- Human Positive Control Primer
- Nuclease-free Water
- MystiCq® Universal PCR Primer
- Poly (A) Polymerase
- microRNA cDNA Reaction Mix
- ReadyScript® Reverse Transcriptase
储存分类代码
10 - Combustible liquids
法规信息
常规特殊物品
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Dynamic changes in global microRNAome and transcriptome reveal complex miRNA-mRNA regulated host response to Japanese Encephalitis Virus in microglial cells.
Kumari B
Scientific Reports, 6, 20263-20263 (2016)
Daniela Baccelli Mendonça et al.
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Regulation of mTOR signaling depends on an intricate interplay of post-translational protein modification. Recently, mEAK-7 (mTOR associated protein, eak-7 homolog) was identified as a positive activator of mTOR signaling via an alternative mTOR complex. However, the upstream regulation of mEAK-7
Weerayut Wongjampa et al.
PloS one, 13(10), e0206644-e0206644 (2018-11-01)
MicroRNAs (miRNAs) are small non-coding RNAs that function to down-regulate gene expression involving in various cellular processes related to carcinogenesis. Recently, miR-22 was identified as a tumor-suppressing miRNA in many human cancers. However, the regulatory mechanism and the specific function
Justin B Collier et al.
Cellular and molecular life sciences : CMLS, 77(18), 3643-3655 (2019-12-25)
Prior studies have established the important role of extracellular signal-regulated kinase 1/2 (ERK1/2) as a mediator of acute kidney injury (AKI). We demonstrated rapid ERK1/2 activation induced renal dysfunction following ischemia/reperfusion (IR)-induced AKI and downregulated the mitochondrial biogenesis (MB) regulator
商品
Real-time polymerase chain reaction allows researchers to estimate the quantity of starting material in a sample. It has a much wider dynamic range of analysis than conventional PCR
实时聚合酶链式反应可用于计算样本的起始材料量。它相比常规PCR具有更大的检测动态范围。
Sigma Imprint RNA 免疫沉淀试剂盒用于从 HeLa 细胞中共纯化人 argonaute 2 (Ago2) 相关 RNA。MicroRNA通过Mysticq 试剂进行逆转录和定量。
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
实验方案
Method for purification, reverse transcription and quantitative PCR for MicroRNAs using Mysticq reagents
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