产品名称
杂交溶液, for Northern and Southern blotting, liquid (2x concentrate)
form
liquid (2x concentrate)
foreign activity
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.
−20°C
Quality Level
General description
Sigma的杂交液是一种2倍即用型杂交缓冲液，适用于Northern和Southern印迹方案。
Preparation Note
Sigma的杂交液含200 μg/mL DNA，溶剂为10倍SSC和2倍Denhardt溶液：
200 μg/mL DNA
2mg牛血清白蛋白(BSA)
2mg Ficoll
2mg聚乙烯吡咯烷酮(PVP)
0.15 M柠檬酸钠
1.5 M NaCl
200 μg/mL DNA
2mg牛血清白蛋白(BSA)
2mg Ficoll
2mg聚乙烯吡咯烷酮(PVP)
0.15 M柠檬酸钠
1.5 M NaCl
Application
杂交液已用于硝酸纤维素膜的杂交。它也已用于DNA杂交。
适合用作Northern和Southern印迹方案的杂交缓冲液。
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
含少量动物源组分生物产品
此项目有
Smooth muscle-specific expression of SV40 large TAg induces SMC proliferation causing adaptive arterial remodeling
Sindermann JR, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 283(6), H2714-H2724 (2002)
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American journal of physiology. Heart and circulatory physiology, 283(6), H2714-H2724 (2002-10-22)
To study the effects of enhanced smooth muscle cell (SMC) proliferation on arterial vessel geometry in the absence of vessel trauma, we developed a transgenic mouse model expressing SV40 large T antigen under control of the 2.3-kb smooth muscle-myosin heavy
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Molecular cancer research : MCR, 2(12), 712-721 (2005-01-07)
Protein serine/threonine kinase CK2 (formerly casein kinase 2) is a ubiquitous protein kinase that plays key roles in cell growth, proliferation, and survival. We have shown previously that its molecular down-regulation induces apoptosis in cancer cells in culture. Here, we
实验方案
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
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