质量水平
形式
liquid (2x concentrate)
异质活性
DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
储存温度
−20°C
一般描述
Sigma的杂交液是一种2倍即用型杂交缓冲液，适用于Northern和Southern印迹方案。
应用
杂交液已用于硝酸纤维素膜的杂交。它也已用于DNA杂交。
适合用作Northern和Southern印迹方案的杂交缓冲液。
组分
Sigma的杂交液含为10倍SSC和2倍Denhardt溶液中的200 μg/mL DNA：
200 μg/mL DNA
2mg牛血清白蛋白（BSA）
2mg聚蔗糖
2mg聚乙烯吡咯烷酮（PVP）
0.15 M柠檬酸钠
1.5 M NaCl
200 μg/mL DNA
2mg牛血清白蛋白（BSA）
2mg聚蔗糖
2mg聚乙烯吡咯烷酮（PVP）
0.15 M柠檬酸钠
1.5 M NaCl
相关产品
产品编号
说明
价格
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
含少量动物源组分生物产品
Sambrook, J., et al.
Molecular Devices, 9-9 (1989)
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Sindermann JR, et al.
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实验方案
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
Northern和Southern印迹简介：两种将大分子转移到膜支持物的常用方法。该文章还提供了Northern印迹和Southern印迹的实验方案。
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