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安全信息

G1N10

Sigma-Aldrich

GenElute™ 哺乳动物基因组DNA小量制备试剂盒

sufficient for 10 purifications

别名:

哺乳动物基因组DNA小量制备, Gen Elute

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5 G
¥870.17
25 G
¥3,624.03
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¥12,155.56

¥870.17

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5 G
¥870.17
25 G
¥3,624.03
100 G
¥12,155.56

About This Item

UNSPSC代码:
41105501
NACRES:
NA.55

¥870.17

请联系客服了解存货情况

用途

sufficient for 10 purifications

储存温度

15-25°C

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N5538SAB4300426N4142
抗硝基酪氨酸 兔抗 affinity isolated antibody, buffered aqueous solution

N0409

抗硝基酪氨酸 兔抗

单克隆抗-3-硝基酪氨酸 小鼠抗 clone 18G4, purified immunoglobulin, buffered aqueous glycerol solution

N5538

单克隆抗-3-硝基酪氨酸 小鼠抗

Anti-NOS1 (Ab-852) antibody produced in rabbit affinity isolated antibody

SAB4300426

Anti-NOS1 (Ab-852) antibody produced in rabbit

抗-神经丝200 兔抗 IgG fraction of antiserum, buffered aqueous solution

N4142

抗-神经丝200 兔抗

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

IgG fraction of antiserum

Quality Level

200

Quality Level

-

Quality Level

100

Quality Level

200

biological source

rabbit

biological source

mouse

biological source

rabbit

biological source

rabbit

Gene Information

human ... NOS1(4842)

Gene Information

human ... NOS1(4842)

Gene Information

human ... NOS1(4842)

Gene Information

human ... NEFH(4744)
mouse ... Nefh(380684)
rat ... Nefh(24587)

technique(s)

immunohistochemistry (frozen sections): 1:1000 using methacarn-fixed, frozen sections of human Alzheimer′s Disease brain, indirect immunofluorescence: 1:1,000 using A549 cells metabolically labeled with 3-nitrotyrosine, western blot: 1:1,000 using nitrated-BSA, indirect ELISA: 1:1,000 using nitrated-BSA, microarray: suitable

technique(s)

western blot: 1:1,000 using mouse and rat brain

technique(s)

indirect immunofluorescence: 1:100-1:200

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:80 using animal cerebellum sections, microarray: suitable, western blot: suitable

一般描述

GenElute哺乳动物基因组DNA纯化试剂盒可以简单、方便地从多种哺乳动物源样本中分离高纯度的大分子量DNA。此类试剂盒使用了为基因组DNA纯化精选的硅胶膜，采用了方便的离心柱形式。

起始材料首先使用含有离液盐的溶液进行裂解，以确保大分子彻底变性。之后加入乙醇，当裂解物离心通过微量离心管时DNA会结合在硅胶膜上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。预期的基因组DNA得率很大程度上取决于所用的起始材料用量及类型。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

纯化所得的基因组DNA可直接用于限制性酶切、PCR、Southern印迹、测序反应等下游应用。

应用

纯化所得的基因组DNA可直接供以下下游应用使用：
  • 限制性内切酶酶切
  • PCR
  • Southern印迹
  • 测序反应
  • 克隆
  • 连接

特点和优势

  • 预期得率：25 μg / 2 x 106个培养细胞；30 μg / 25 mg组织
  • 洗脱体积：200 - 400 μl
  • 所需时间：细胞裂解后20分钟
  • A260/A280比率：1.6 - 1.9
  • 是否需要机械式均质化：否

原理

起始材料首先使用含有离液盐的溶液进行裂解，以确保大分子彻底变性。之后加入乙醇，裂解物离心通过微量离心管时，DNA会结合到硅胶模上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。预期的基因组DNA得率很大程度上取决于所用的起始材料用量及类型。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。

其他说明

更多信息，请见www.sigma-aldrich.com/genomicdna。

法律信息

GenElute is a trademark of Sigma-Aldrich Co. LLC

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • C2112Column Preparation Solution化学品安全说明书
  • P2308Proteinase K from Tritirachium album, lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology化学品安全说明书
  • R6148RNase A solution化学品安全说明书

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警示用语：

Danger

危险分类

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

8A - Combustible corrosive hazardous materials

闪点(°F)

483.8 °F

闪点(°C)

251 °C

法规信息

常规特殊物品
  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    SLCL7191
    SLCK8229
    SLCF6078
    SLCC7185
    SLCB3846

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    We have previously shown that in vitro transduction with bovine adeno-associated viral (BAAV) vectors restores connexin expression and rescues gap junction coupling in cochlear organotypic cultures from connexin-deficient mice that are models DFNB1 nonsyndromic hearing loss and deafness. The aims
    Michal Kirshner et al.
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    Pronounced neuropathology is a feature of ataxia-telangiectasia (A-T) and Nijmegen breakage syndrome (NBS), which are both genomic instability syndromes. The Nbs1 protein, which is defective in NBS, is a component of the Mre11/RAD50/NBS1 (MRN) complex. This complex plays a major
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