General description
起始材料首先使用含有离液盐的溶液进行裂解，以确保大分子彻底变性。之后加入乙醇，当裂解物离心通过微量离心管时DNA会结合在硅胶膜上。在洗涤去除污染物后，将DNA放在200 μL的Tris-EDTA溶液中洗脱。预期的基因组DNA得率很大程度上取决于所用的起始材料用量及类型。通过本试剂盒纯化所得的DNA的A260/A280比率介于1.6-1.9之间，最大长度可达50 kb。
纯化所得的基因组DNA可直接用于限制性酶切、PCR、Southern印迹、测序反应等下游应用。
Application
- 限制性内切酶酶切
- PCR
- Southern印迹
- 测序反应
- 克隆
- 连接
Biochem/physiol Actions
Features and Benefits
- 预期得率：25 μg / 2 x 106个培养细胞；30 μg / 25 mg组织
- 洗脱体积：200 - 400 μl
- 所需时间：细胞裂解后20分钟
- A260/A280比率：1.6 - 1.9
- 是否需要机械式均质化：否
Other Notes
Legal Information
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signalword
Danger
target_organs
Respiratory system
flash_point_f
483.8 °F
flash_point_c
251 °C
wgk
WGK 3
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - ED ENV 1 - Eye Dam. 1 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
存储类别
10 - Combustible liquids
法规信息
此项目有
实验方案
GenomePlex® Whole Genome Amplification efficiently extracts DNA from animal samples for genomic analysis.
This protocol provides a simple and convenient method to isolate, amplify, and purify genomic DNA from buccal swabs. Buccal swabs are a convenient method of acquiring a DNA sample.
The GenElute Mammalian Genomic DNA Purification Kit Protocol describes the isolation of pure, high molecular weight DNA from a variety of mammalian sources.
商品
Simple DNA/RNA purification methods aid genome analysis from various sources, enhancing research efficiency.
相关内容
GenElute Mammalian Genomic DNA Purification Kit isolates pure DNA from mammalian cells using a silica-based membrane in spin columns.
GenomePlex®全基因组扩增是从动物样品中提取DNA的方法。GenomePlex®产品已用于扩增鸡、猪、牛、鱼和虾来源的基因组DNA。
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| G1N10-1KT | 04061833628546 |