Quality Level
biological source
non-animal source
type
Type 400
form
powder, spray-dried
optical activity
[α]20/D 56.5°, c = 1% (w/v) in water
technique(s)
tissue culture: suitable
color
white to off-white
solubility
H2O: 100 mg/mL, clear to slightly hazy, colorless to faintly yellow
suitability
suitable for component for culture media
storage temp.
room temp
General description
Ficoll 400是由蔗糖和环氧氯丙烷共聚而成的一种高度分支化的聚合物。 Ficoll 400是完全非离子性的。 由于其含有丰富的羟基，Ficoll 400具有很强的亲水性和水溶性。 Ficoll 400最常见的应用是作为密度梯度介质，用于真核细胞、细胞器和细菌细胞的分离和分离。密度范围可达1.2 g/ml。 它还被用于一系列其他应用中。
Application
用于细胞分离和细胞器分离。
Ficoll® PM 400与泛影酸(Paque)一起使用，产生Ficoll-Paque，是一种用于分离细胞和细胞器的密度混合物，特别是血液来源的淋巴细胞和单核细胞。它可用于组织工程和大分子构象研究，用于大分子拥挤（MMC）系统和配置的开发、评估和使用。Ficoll PM-400可应用于细胞冷冻-干燥研究。
Features and Benefits
由于高分子量和低含量的透析性材料，Ficoll对细胞膜的渗透性比蔗糖低得多。 因此，细胞在梯度Ficoll下的聚集密度要低于梯度蔗糖。Ficoll由于其低膜渗透性和低渗透压，通常能较好地保存细胞功能和形态。
Preparation Note
水的浓度可达到50% (w/v)。 Ficoll应在持续搅拌下缓慢加入。
Sigma测试Ficoll 400的溶解度，其1g溶于10ml去离子水中时呈透明到微模糊，无色到淡黄色的溶液。 Ficoll在碱性和中性溶液中稳定。当pH值低于3时，它会迅速水解，尤其是在高温下。 Ficoll可在110oC的中性pH下高压灭菌30分钟。 应避免使用强氧化性和还原性试剂。
Sigma测试Ficoll 400的溶解度，其1g溶于10ml去离子水中时呈透明到微模糊，无色到淡黄色的溶液。 Ficoll在碱性和中性溶液中稳定。当pH值低于3时，它会迅速水解，尤其是在高温下。 Ficoll可在110oC的中性pH下高压灭菌30分钟。 应避免使用强氧化性和还原性试剂。
Legal Information
Ficoll is a registered trademark of Cytiva
存储类别
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| F4375-25G | 04061833617663 |
| F4375-500G | 04061833617670 |
| F4375-100G | 04061833617649 |
| F4375-250G | 04061842383757 |
| F4375-10G | 04061833617656 |
| F4375-50G | 04061842383764 |