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形式
liquid
特点
hotstart
浓度
10 mM (each dNTP)
颜色
colorless
运输
dry ice
储存温度
−20°C
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一般描述
我们很荣幸推出新的改进型 CleanAmp dNTP。 CleanAmp dNTP 是改性核苷三磷酸,可以阻止 DNA 聚合酶核苷酸掺入。 在典型的热启动 PCR 循环条件下,CleanAmp dNTP 通过初始加热步骤和随后的变性步骤激活。 这一过程限制了每个 PCR 周期中激活的 dNTP 的数量,允许对所需产物进行特异性和效率更高的扩增,同时减少甚至完全避免错误引物或引物二聚体的形成。 CleanAmp dNTP 为各种基于 PCR 的应用提供了比热启动酶更经济的解决方案。
CleanAmp dNTPs 是 TriLink BioTechnologies 公司的一款产品。CleanAmp dNTPs 是修饰的核苷三磷酸,可阻断 DNA 聚合酶核苷酸掺入。CleanAmp dNTPs 通过典型热启动 PCR 循环条件中的初始加热步骤和随后的变性步骤激活。该过程限制了每个 PCR 循环期间激活的 dNTP 的量,从而更特异和有效地扩增目标产物,并减少甚至消除错配或引物二聚体。CleanAmp dNTP 为各种基于 PCR 的应用提供了比热启动酶更经济的解决方案。
应用
- 用于需要减少非特异性扩增的 PCR 扩增
- 用于多重 PCR
- 用于减少引物二聚体
- 在任何 PCR 反应中替代 dNTP
包装
2 μmoles:1 小瓶,200 μL,每种 dNTP 10 mM 溶于 50 mM 甘氨酸溶液
10 μmoles:1 小瓶,1000 μL,每种 dNTP 10 mM 溶于 50 mM 甘氨酸溶液
10 μmoles:1 小瓶,1000 μL,每种 dNTP 10 mM 溶于 50 mM 甘氨酸溶液
法律信息
CleanAmp™ dNTP 由 TriLink BioTechnologies 公司提供给买方,具有不可转让的权力,无论买方是学术、非营利或营利机构,买方可以使用购买的 CleanAmp™ dNTP 进行的内部研究。CleanAmp™ dNTP 不得用于人类疾病治疗或商业临床诊断。无论使用机构的学术或非营利状态如何,将 CleanAmp™ dNTP 应用于任何商业用途均需要获得许可证。有关 CleanAmp™ dNTP 商业许可证的信息可以向 TriLink BioTechnologies 公司索取。买方不得使用 CleanAmp™ dNTP 支持在世界上任何国家提交针对 CleanAmp™ dNTP 权利要求的专利申请或未经 TriLink BioTechnologies 公司批准使用该产品。如果您未能遵守本协议的这些条款和条件,买方拥有和使用 CleanAmp™ dNTP 的权利将立即终止。
CleanAmp is a trademark of TriLink BioTechnologies, Inc.
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
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Nucleic acid testing (NAT) is valuable for screening blood donors for occult hepatitis B virus (HBV) infection and infection during the window period in countries where HBV is endemic, such as China. An "in-house" NAT (Triplex NAT) was developed for
Nucleic acids research, 39(14), 5893-5906 (2011-04-12)
Parental genomic imprinting at the Igf2/H19 locus is controlled by a methylation-sensitive CTCF insulator that prevents the access of downstream enhancers to the Igf2 gene on the maternal chromosome. However, on the paternal chromosome, it remains unclear whether long-range interactions
Nucleic acids symposium series (2004), (52)(52), 259-260 (2008-09-09)
Several 3'-ether and 3'-ester derivatives of 2'-deoxyribonucleoside 5'-triphosphates (dNTPs) were prepared. These dNTP derivatives were not substrates for DNA polymerase and did not support primer extension at room temperature. However, by short pre-heating to 95 degrees C in PCR buffer
Methods in molecular biology (Clifton, N.J.), 630, 301-318 (2010-03-20)
Hot Start activation approaches are increasingly being used to improve the performance of PCR. Since the inception of Hot Start as a means of blocking DNA polymerase extension at lower temperatures, a number of approaches have been developed that target
Current protocols in molecular biology, Chapter 15, Unit 15-Unit 15 (2009-10-10)
Hot-start PCR is a technique that improves PCR performance by reducing nonspecific amplification during the initial setup stages of the PCR. This unit describes hot-start PCR protocols which utilize primers containing temperature-sensitive modifications. The introduction of 4-oxo-tetradecyl (OXT) phosphotriester groups
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