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主要文件

安全信息

DNTPCA1

Sigma-Aldrich

CleanAmp dNTP

Hot-start dNTP mix for improved PCR

别名:

dNTP, dNTP 混合物, 脱氧核苷酸

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About This Item

UNSPSC代码:
41106305

产品DNTPCA1监管中,请联系销售询价或购买 联系客户支持

表单

liquid

特点

hotstart

浓度

10 mM (each dNTP)

颜色

colorless

运输

dry ice

储存温度

−20°C

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一般描述

CleanAmp dNTPs 是 TriLink BioTechnologies 公司的一款产品。CleanAmp dNTPs 是修饰的核苷三磷酸,可阻断 DNA 聚合酶核苷酸掺入。CleanAmp dNTPs 通过典型热启动 PCR 循环条件中的初始加热步骤和随后的变性步骤激活。该过程限制了每个 PCR 循环期间激活的 dNTP 的量,从而更特异和有效地扩增目标产物,并减少甚至消除错配或引物二聚体。CleanAmp dNTP 为各种基于 PCR 的应用提供了比热启动酶更经济的解决方案。
我们很荣幸推出新的改进型 CleanAmp dNTP。 CleanAmp dNTP 是改性核苷三磷酸,可以阻止 DNA 聚合酶核苷酸掺入。 在典型的热启动 PCR 循环条件下,CleanAmp dNTP 通过初始加热步骤和随后的变性步骤激活。 这一过程限制了每个 PCR 周期中激活的 dNTP 的数量,允许对所需产物进行特异性和效率更高的扩增,同时减少甚至完全避免错误引物或引物二聚体的形成。 CleanAmp dNTP 为各种基于 PCR 的应用提供了比热启动酶更经济的解决方案。

应用

  • 用于需要减少非特异性扩增的 PCR 扩增
  • 用于多重 PCR
  • 用于减少引物二聚体
  • 在任何 PCR 反应中替代 dNTP

包装

2 μmoles:1 小瓶,200 μL,每种 dNTP 10 mM 溶于 50 mM 甘氨酸溶液
10 μmoles:1 小瓶,1000 μL,每种 dNTP 10 mM 溶于 50 mM 甘氨酸溶液

法律信息

CleanAmp dNTP 由 TriLink BioTechnologies 公司提供给买方,具有不可转让的权力,无论买方是学术、非营利或营利机构,买方可以使用购买的 CleanAmp dNTP 进行的内部研究。CleanAmp dNTP 不得用于人类疾病治疗或商业临床诊断。无论使用机构的学术或非营利状态如何,将 CleanAmp dNTP 应用于任何商业用途均需要获得许可证。有关 CleanAmp dNTP 商业许可证的信息可以向 TriLink BioTechnologies 公司索取。买方不得使用 CleanAmp dNTP 支持在世界上任何国家提交针对 CleanAmp dNTP 权利要求的专利申请或未经 TriLink BioTechnologies 公司批准使用该产品。如果您未能遵守本协议的这些条款和条件,买方拥有和使用 CleanAmp dNTP 的权利将立即终止。
CleanAmp is a trademark of TriLink BioTechnologies, Inc.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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  • 历史批次信息供参考:

    分析证书(COA)

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    Xinglong Xiao et al.
    Journal of virological methods, 187(2), 357-361 (2012-11-13)
    Nucleic acid testing (NAT) is valuable for screening blood donors for occult hepatitis B virus (HBV) infection and infection during the window period in countries where HBV is endemic, such as China. An "in-house" NAT (Triplex NAT) was developed for
    Franck Court et al.
    Nucleic acids research, 39(14), 5893-5906 (2011-04-12)
    Parental genomic imprinting at the Igf2/H19 locus is controlled by a methylation-sensitive CTCF insulator that prevents the access of downstream enhancers to the Igf2 gene on the maternal chromosome. However, on the paternal chromosome, it remains unclear whether long-range interactions
    Elena Hidalgo Ashrafi et al.
    Current protocols in molecular biology, Chapter 15, Unit 15-Unit 15 (2009-10-10)
    Hot-start PCR is a technique that improves PCR performance by reducing nonspecific amplification during the initial setup stages of the PCR. This unit describes hot-start PCR protocols which utilize primers containing temperature-sensitive modifications. The introduction of 4-oxo-tetradecyl (OXT) phosphotriester groups
    Franck Court et al.
    Genome biology, 12(5), R42-R42 (2011-05-17)
    Despite its critical role for mammalian gene regulation, the basic structural landscape of chromatin in living cells remains largely unknown within chromosomal territories below the megabase scale. Here, using the 3C-qPCR method, we investigate contact frequencies at high resolution within
    Inna Koukhareva et al.
    Nucleic acids symposium series (2004), (52)(52), 259-260 (2008-09-09)
    Several 3'-ether and 3'-ester derivatives of 2'-deoxyribonucleoside 5'-triphosphates (dNTPs) were prepared. These dNTP derivatives were not substrates for DNA polymerase and did not support primer extension at room temperature. However, by short pre-heating to 95 degrees C in PCR buffer

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