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CMC0008

Sigma-Aldrich

STEADY 化学感受态细胞 

Escherichia coli, rod shaped

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About This Item

UNSPSC代码:
12352200

product name

STEADY 化学感受态细胞 , for plasmid production using unstable DNA

生物来源

Escherichia coli

等级

for molecular biology

形式

buffered aqueous solution

生长模式

adherent or suspension

形态学

rod shaped

技术

microbiological culture: suitable

细胞转化

competent cell type: chemically competent
transformation efficiency: >1 x 108 cfu/μg

运输

dry ice

储存温度

−70°C

一般描述

The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.

Genotype

recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

特点和优势

  • Clone unstable sequences with reliable results
  • Available as chemically competent or electrocompetent cells
  • Your choice of two packaging formats
  • High efficiency: over 1 × 108 cfu/μg (chemi) or 1 × 1010 cfu/μg (electro)
  • Excellent value

组分

  • STEADY chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning


储存分类代码

10 - Combustible liquids

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