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Merck
CN

C1562

Sigma-Aldrich

梯度降温盒,Nalgene® Mr. Frosty

H × diam. 86 mm × 117 mm

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别名:
freezer container, freezer storage, storage container
UNSPSC代码:
41122800
NACRES:
NB.22

包装

pack of 1 ea

制造商/商品名称

Nalgene 5100-0001

高度 × 直径

86 mm × 117 mm

相关类别

一般描述

聚碳酸酯降温盒,蓝色高密度聚乙烯盖,白色高密度聚乙烯冻存管支架和泡沫衬垫。

为成功实现细胞冷冻保存提供所需的关键的、可重复的、1°C/分钟的冷却速率。方便用于任何机械冷冻设备。降温盒上印有图示说明。只需要异丙醇。刚性冻存管支架确保冻存管不接触酒精,防止芯吸污染。冻存管支架漂浮,方便水浴解冻。具有数字标记,方便识别。

所有部件均可承受反复冻融。可叠起堆放。

应用

Nalgene Mr. Frosty梯度降温盒可用于冷冻植物细胞、原生质体 和PBMC(外周血单核细胞)

相容性

可容纳18个1.2和2.0 mL冻存管

法律信息

Nafion is a trademark of The Chemours Company FC, LLC
Nalgene is a registered trademark of Thermo Fisher Scientific or its subsidiaries

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What alcohol should I use with Mr. Frosty, Product C1562 (Freezing container, Nalgene®)?

    We recommend using 2-propanol, commonly known as isopropanol. Among our product listings for 2-propanol we offer Product No. 190764.

  4. How do I freeze my cells in a Nalgene® Mr. Frosty, freezing container, product C1562?

    Listed below are instructions for the Nalgene® Mr. Frosty freezing container.Fill the container with room-temperature 100% isopropyl alcohol to the indicated fill line and discard the foam insert and tube holder. Insert your sample tubes into the tube holder and place the Mr. Frosty container in -70°C freezer for a minimum of 4 hours. Remove frozen tubes and place in long term storage freezer, at or below -130°C.Note: When not in use, the Mr. Frosty container should be stored at room temperature.

  5. What is the rate of cooling of the cell suspension in Product C1562, Freezing container, Nalgene®?

    The rate of cooling of 1°C/minute is based on the use of isopropanol as the alcohol bath.

  6. Can I use Product C1562, Freezing container, Nalgene®, with 5 mL freezing vials?

    Product No. C1562 is designed for use with 1.2 and 2 mL freezing vials.  It cannot be used with 5 mL freezing vials. Nalgene no longer offers the Mr. Frosty in the 5 mLsize.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Helen A Fletcher et al.
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Arend F van Peer et al.
Applied and environmental microbiology, 75(5), 1243-1247 (2008-12-31)
Phleomycin is mutagenic by introducing double-strand breaks in DNA. The ble gene of Streptoalloteychus hindustanus, which confers resistance to this substance, is widely used as a selection marker for transformation. Schizophyllum commune grows on 25 microg of phleomycin ml(-1) after
Vanessa Cristina de Oliveira et al.
PloS one, 15(7), e0235856-e0235856 (2020-07-11)
Gene editing in large animal models for future applications in translational medicine and food production must be deeply investigated for an increase of knowledge. The mitochondrial transcription factor A (TFAM) is a member of the HMGB subfamily that binds to
Ji-Suk Cho et al.
Applied microbiology and biotechnology, 73(6), 1470-1476 (2006-10-06)
Transgenic suspension cells of Oryza sativa L. cv. Dongjin utilized as a host for producing recombinant human cytotoxic T-lymphocyte antigen 4-immunoglobulin (hCTLA4Ig) were preserved in liquid nitrogen (-196 degrees C) after slow prefreezing in a deep freezer (-70 degrees C).
Lise M van Wijk et al.
Cancers, 12(10) (2020-10-03)
Recent studies have shown that the efficacy of PARP inhibitors in epithelial ovarian carcinoma (EOC) is related to tumor-specific defects in homologous recombination (HR) and extends beyond BRCA1/2 deficient EOC. A robust method with which to identify HR-deficient (HRD) carcinomas

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实验方案

Cell culture protocol for high cell viability freezing using cryopreservation reagents like DMSO. Free handbook download.

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