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应用
BCIP®/NBT液体底物系统用于：
- 对骨髓基质细胞中的碱性磷酸酶（ALP）进行染色
- 用作Western 印迹检测的底物，检测基质金属蛋白酶-2特异性抗体
- 作为酶联免疫斑点（ELISpot）测定中的底物，测定链霉抗生物素蛋白碱性磷酸酶
碱性磷酸酶的即用型沉淀底物系统。该底物系统能够产生不溶的NBT二甲双胍终产物，其颜色为蓝色至紫色并且可以在视觉上观察到。使用BCIP/NBT的免疫组织化学染色需要有机封固剂，并且可以用核固红或亮绿复染。BCIP/NBT通常比固红更灵敏。 不建议用于ELISA（微孔）检测。
注意
建议将本品储存在4-8°C之间。请勿冷冻。本品可在室温下储存最长一年。如果产生沉淀，可将溶液轻轻涡旋或过滤。
法律信息
BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Commensal microbes induce serum IgA responses that protect against polymicrobial sepsis
Wilmore JR, et al.
Cell host & microbe, 23(3), 302-311 (2018)
Nana H Overgaard et al.
Vaccine, 35(42), 5629-5636 (2017-09-10)
The relationship between the antigen dose and the quality of an immune response generated upon immunization is poorly understood. However, findings show that the immune system is indeed influenced by the antigen dose; hence underlining the importance of correctly determining
Epigenetic regulation of Runx2 transcription and osteoblast differentiation by nicotinamide phosphoribosyltransferase
Ling M, et al.
Cell & Bioscience, 7(1), 27-27 (2017)
Salma E Jiménez-Badillo et al.
Scientific reports, 7(1), 13351-13351 (2017-10-19)
The Catsper1 gene, whose expression is restricted to male germ cells, has great importance in reproductive biology because of its function in sperm motility and fertilization. We previously reported that the promoter of this gene has transcriptional activity in either
Jungho Jang et al.
Scientific reports, 8(1), 7211-7211 (2018-05-10)
The conversion of carbon dioxide to formate is a fundamental step for building C1 chemical platforms. Methylobacterium extorquens AM1 was reported to show remarkable activity converting carbon dioxide into formate. Formate dehydrogenase 1 from M. extorquens AM1 (MeFDH1) was verified
商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。
实验方案
5-溴-4-氯-3-吲哚基磷酸盐（BCIP）/ 硝基蓝四唑（NBT）是一种理想的不溶性底物，可与碱性磷酸酶一起使用。该系统产生蓝紫色产物。可以目视观察到强烈的颜色，非常稳定，并且在暴露于光时不褪色。
BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.
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