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安全信息

A9647

Sigma-Aldrich

牛血清白蛋白 来源于牛血清

heat shock fraction, pH 7, ≥98%

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别名:
BSA, 牛白蛋白, 牛血清白蛋白
CAS号:
9048-46-8
EC 号:
232-936-2
MDL编号:
MFCD00130384
UNSPSC代码:
12352202
NACRES:
NA.27

生物来源

bovine

质量水平

300

检测方案

≥98%

形式

lyophilized powder

分子量

~66 kDa

纯化方式

heat shock fractionation

来源

USA origin

技术

immunocytochemistry: suitable

杂质

≤5.0% Loss on drying

缺失

≤5%

pH值（酸碱度）

7

溶解性

water: soluble (40 mg/ml)

UniProt登记号

P02769

异质活性

BT virus, none detected
VSV virus, none detected

储存温度

2-8°C

基因信息

bovine ... ALB(280717)

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相关类别

一般描述

牛血清白蛋白(BSA)是一种蛋白质，最关键的生物学作用是调节血液的胶体渗透压。BSA广泛用于各种研究和应用。BSA由583个氨基酸组成， 是一种水溶性蛋白质，分子量为66,430 Da。六个α-螺旋形成了BSA的三个同源结构域。它可依赖于pH进行可逆的构象异构化。蛋白的天然结构在加热时会变得具有反应性和灵活性。

应用

牛血清白蛋白(BSA)可用于多种应用，例如以下应用：
  • BSA可用于细胞培养，例如，作为少突胶质细胞培养基的补充剂。
  • 在免疫组化实验中，BSA可稀释一抗。
  • BSA可作为Bradford法测蛋白质浓度的标准品。
  • 在免疫荧光研究中，BSA可用在封闭和透化缓冲液中。

生化/生理作用

BSA的某些构象和初级序列表位是人类对牛肉和牛奶过敏中的可疑过敏原。

制备说明

热激分级分离法制取
血清白蛋白可能也会被称为Fraction V。该命名惯例取自使用冷乙醇沉淀对血清蛋白进行分级的原始Cohn法。 血清白蛋白可在采用Cohn′法的第五乙醇分级中发现。 从此，有些人便开始使用“Fraction V”这一术语来描述血清白蛋白，无论使用的是什么制备方法。 也有人在使用该术语来描述通过乙醇分级方法而纯化得到血清白蛋白，而这种方法是相对于原始的Cohn法一直在得到高度的改良。Sigma-Aldrich生产和分销从各种主要方法纯化得到的血清白蛋白，包括真正的Cohn分级法、改良的乙醇分级方法、热休克和色谱法。 更多的纯化步骤包括结晶或木炭过滤。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

新产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the molecular weight of Product A9647, Albumin from bovine serum?

    The molecular weight of BSA is 66,430.

  4. How can the concentration of a solution of Product A9647, Albumin from bovine serum, be quantified?

    The concentration of a solution of BSA can be measured by using the extinction coefficient (E1%) of 6.67. The 1% refers to a solution that is 1% weight-to-volume (i.e., 10 mg/mL).

  5. What is the pH of a solution of Product A9647, Albumin from bovine serum?

    The pH of a 1% solution is approximately 7.0, according to the product specifications.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Mohammad Bahrami et al.
Scientific reports, 9(1), 11739-11739 (2019-08-15)
Defining oocyte in vitro maturation (IVM) conditions allows for improved reproducibility and efficiency of bovine embryo production. IVM conditions for bovine oocytes have been extensively studied, but beneficial effects of individual supplements remain controversial. This study compared methods of cumulus
Characterization of an Exo-beta-1,3-Glucanase Produced by Pichia anomala Strain K, Antagonist of Botrytis cinerea on Apples.
Jijakli MH et al.
Phytopathology, 88, 335-335 (1998)
Down-regulation of seladin-1 increases BACE1 levels and activity through enhanced GGA3 depletion during apoptosis.
Sarajarvi T et al.
The Journal of Biological Chemistry, 284, 34433-34433 (2009)
E-cadherin, MMP-2, and MMP-9 as prognostic markers in penile cancer: analysis of 125 patients.
Campos RS et al.
Urology, 67, 797-797 (2006)
Corrigendum: Formation and reshuffling of disulfide bonds in bovine serum albumin demonstrated using tandem mass spectrometry with collision-induced and electron-transfer dissociation.
Rombouts I et al.
Scientific Reports, 5 (2015)
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