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Merck
CN
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文件

21888

Sigma-Aldrich

5(6)-羧基二乙酸荧光素 N-琥珀酰亚胺酯

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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别名:
CFSE
经验公式（希尔记法）:
C29H19NO11
CAS号:
150347-59-4
分子量:
557.46
MDL编号:
MFCD00037466
UNSPSC代码:
12352108
PubChem化学物质编号:
57647951
NACRES:
NA.32

产品线

BioReagent

质量水平

200

检测方案

≥90% (HPLC)

形式

solid

mp

152-154 °C (lit.)

溶解性

DMF: soluble
DMSO: soluble

荧光

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

适用性

suitable for fluorescence

储存温度

−20°C

SMILES字符串

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3

InChI key

LZFDYQHOQDFMOA-UHFFFAOYSA-N

相关类别

一般描述

5-（和-6）-羧基荧光素二乙酸酯N-琥珀酰亚胺酯（CFSE）是一种荧光染料，可透膜。它通过与细胞内分子之间进行共价结合来标记细胞。CFSE可以用于追踪淋巴细胞的迁移和增殖。

应用

5(6)-羧基二乙酸荧光素 N-琥珀酰亚胺酯（CESE）是一种胺反应性荧光探针，可用于对细胞染色，特别是免疫细胞，以便在流式细胞术等应用中进行分析。经过脱乙酰化形成绿色荧光偶联物。
5-双乙酸荧光素N-琥珀酰亚胺酯可用于：
  • 标记细胞以进行流式细胞术
  • 在淋巴细胞增殖测定中
  • 确定细胞内或叶绿体中细胞内的pH值
  • 标记亚马逊利什曼原虫 和大利什曼原虫

作为胺反应性标记物用于细胞的长期追踪。经过脱乙酰化形成绿色荧光偶联物。

生化/生理作用

5（6）-羧荧光素二乙酸酯N-琥珀酰亚胺酯可用作酯酶活性指标。

包装

无底玻璃瓶。内容物在插入式融锥内。

相关产品

产品编号
说明
价格

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. At what concentration is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, soluble in DMSO?

    We have found 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester to be soluble in DMSO at a concentration of 1 mg/mL.

  4. What is the ratio between the (5) and (6) isomer of Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester?

    Our Quality Control group does not routinely report the ratio; however, it is very likely that both isomers are present.

  5. Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, light sensitive?

    Yes. The powder and solutions (especially solutions) should be kept protected from ambient light whenever possible.

  6. Is Product 21888, 5(6)-Carboxyfluorescein diacetate N-succinimidyl ester, fluorescent? Are conjugates of this compound fluorescent?

    No. This compound and conjugates of this compound become fluorescent only when acted upon by an enzyme such as esterase. The enzyme removes the acetate groups.

  7. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  8. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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