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特异性
过氧化物酶底物适用于免疫印迹和免疫组织染色实验操作。该底物能够产生不溶的终产物,其颜色为棕色并且可以在视觉上观察到。
应用
3,3′-二氨基联苯胺四盐酸盐已被用于免疫组化。
用于分光光度法测定硒的试剂。
生化/生理作用
3,3′-二氨基联苯胺四盐酸盐是一种生色底物,通常用于免疫过氧化物酶测试。
重悬
将1片片剂溶于15ml Tris缓冲盐水(pH 7.6)中。 加入12 μl新鲜的30%过氧化氢。可通过调节DAB和过氧化氢浓度以适用于各种应用。
警示用语:
Danger
危险分类
Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2
WGK
WGK 3
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
危险化学品
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商品
NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.
硝基蓝四唑(NBT)可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐(BCIP)一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物,其颜色为蓝至紫色,可以通过视觉观察到。
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