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形式
solution
质量水平
用途
1.0 mL sufficient for 100 mini-gels
环保替代产品特性
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
浓度
10,000 × in DMSO
技术
PCR: suitable
环保替代产品分类
储存温度
−20°C
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一般描述
应用
- dsDNA定量
- 聚合酶链式反应（PCR），用于DNA染色
- Comet测定技术
- 评估精子膜的完整性
- 扩增DNA 目视检查（环介导等温扩增（LAMP）法）
- 用作流式细胞术中的荧光染料
- 实时定量逆转录聚合酶链式反应（qRT-PCR），用于逆转录cDNA染色
特点和优势
- 用于在电泳后对琼脂糖或聚丙烯酰胺凝胶中的双链DNA染色的超灵敏染色剂
- 它还可以检测变性琼脂糖/甲醛和聚丙烯酰胺/尿素凝胶中的ssDNA和RNA，无需任何预洗涤步骤
- 在 Ames 试验中，它的致突变性比溴化乙锭小
- 它对寡核苷酸的检测灵敏度是溴化乙锭的 50-100 倍
- 适用于 DNA 数量有限的许多应用
- SYBR® Green I 与 DNA 的结合不会抑制许多常见限制性内切酶的活性，包括 Hind III 和 EcoR I
- 这种染色剂无需通过凝胶酶解和连接技术去除
- SYBR Green I 是溴化乙锭染色的绿色替代产品
储存及稳定性
法律信息
WGK
WGK 3
闪点(°F)
closed cup
闪点(°C)
closed cup
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Will Product S9430, SYBR® Green I nucleic acid gel stain, stain DNA that contains deaza-G modified nucleotides in place of guanisine?
Product S9430, SYBR® Green I nucleic acid gel stain, will poorly stain DNA containing deaza-G modified nucleotides in place of guanisine. A way around this issue would be to use a mixture of deaza-G modified nucleotides with guanisine. This will allow for better staining.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
detection of Tritrichomonas foetus
商品
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
实验方案
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
面向全基因组扩增(WGA)的SeqPlexDNA扩增试剂盒(SEQXE)专用于对极小量或降解/高度片段化的DNA进行下一代测序(NGS)。
Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.
WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias
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