Validated Cell Types for the Scepter™ 3.0 Cell Counter
The Scepter™ counter has been shown to be accurate over a range of cell types and sizes. See below for a list of mammalian and other cell types that have been validated for Scepter™ counting.
Measured
• Recommended based on size
• Customer Validated
• Merck Scepter™ 2.0 Validated
• Merck Scepter™ 3.0 Validated
1 histograms may be cut-off at high concentrations, dilution may be needed
| Cell Type | size (μm) | 40 μm sensor | 60 μm sensor |
|---|---|---|---|
| 2102 Ep | 15-19 | • | |
| 454 beads | 28 | • | |
| A10 | 22 - 24 | • | |
| A172 | 15 | • | |
| A253 | 14-18 | • | |
| A375 | 16 | • | |
| A431 | 15-17 | • | |
| A549 | 11-15 | • | |
| Algae (various) | 7-9 | • | • |
| B35 | 13-16 | • | • |
| B Cells | 6-11 | • | |
| C2C12 | 12 | • | • |
| C305 | 12-14 | • | • |
| C6 | 12-13 | • | |
| CA46 | 10-12 | • | • |
| Caco-2 | 17 | •• | |
| CHO | 13-17 | •• | |
| COS-1 | 12 | • | |
| Cos-7 | 15 | • | |
| D283 | 12 | • | |
| Daudi | 10-12 | • | • |
| DU-145 | 15-17 | • | |
| EL4 (Blood Murine Lymphoblast) | 12-14 | • | • |
| Epithelia | 14-15 | • | |
| Epithelia MDA-MB-231 | 16-17 | • | • |
| HCC827 (Human lung adenocarcinoma) | 18 | • | |
| HCT-116 | 10 | • | • |
| HEK293 | 11-16 | •• | |
| HeLa | 12-15 | • | •• |
| HepG2 | 12-17 | •1 | •• |
| HFF | 18-20 | • | |
| Hs27 | 14 | • | • |
| HT-1080 (fibrosarcoma) | 14-16 | •• | |
| HT-29 | 11 | • | |
| HUH7 (Hepatoma) | not available | ||
| Human ES Cells | 9-12 | • | |
| HUVEC | 14-15 | • | |
| Hybridoma | 13-14 | • | |
| IMR-32 | 12-14 | • | • |
| IMR-90 | 15 | • | • |
| iPSCs | 16-17 | •1 | • |
| Jurkat | 12-13 | • | •• |
| K562 | 22 | • | |
| KB | 14 | • | |
| KG-1 | 10-13 | • | • |
| L6 | 14-16 | • | |
| LNCaP | 15-16 | • | |
| Luminex®beads | 5-6 | • | |
| MCF7 | 15-21 | •• | |
| MDCK | 13-15 | • | •• |
Measured
• Recommended based on size
• Customer Validated
• Merck Scepter™ 2.0 Validated
• Merck Scepter™ 3.0 Validated
1 histograms may be cut-off at high concentrations, dilution may be needed
| Cell Type | size (μm) | 40 μm sensor | 60 μm sensor |
|---|---|---|---|
| Meg-01 | 16-17 | • | |
| MG-63 | 15-17 | • | |
| Mouse ES Cell | 5-13 | • | • |
| MN9D (Mouse Dopaminergic Neuronal) | 15 | • | |
| MSc (Mesenchymal Stem) | 15-16 | ••1 | • |
| MRC-5 | 18 | • | |
| NCI-H146 | 10-13 | • | |
| NIH 3T3 | 15 | • | •• |
| NTERA2, clone D1 | 13 | • | • |
| OK | 17-18 | • | |
| PBMCs | 7-12 | • | |
| PC12 | 9-15 | • | • |
| Primary Astrocytes | 7 | • | |
| Primary Neuronal Cell | Varies | • | |
| Raji | 12-15 | • | • |
| Ramos | 11-12 | • | • |
| Rat Dorsal Root Ganglion Cells | 20-30 | • | |
| Rat Whole Blood | 4.6 | •• | |
| Red Blood Cells | 5-7 | • | |
| Rat Neural Stem Cell (RNSC) | 11-13 | • | |
| RAW 264.7 Cell Line murine macrophage from blood | 11-13 | • | • |
| RBL | 11-13 | • | • |
| RIN-mF5 | 13-14 | • | |
| S462 (Human Peripheral Nerve Sheath Tumor) | 16 | • | |
| SF9 | 13 | • | |
| SH-SY5Y | 12 | • | |
| Sk-Br-3 | 15-20 | • | |
| SK-MEL-28 | 17-19 | • | |
| SK-N-MC | 14-15 | • | |
| SK-N-SH | 14-15 | • | |
| Splenocytes | 7-9 | • | |
| SW-480 | 15 | • | |
| SW-620 | 13-14 | • | • |
| T84 | 14-18 | • | |
| T98G | 17 | • | |
| T cells: naïve | 5-7 | • | |
| T cells: CD3+ | 11-12 | • | • |
| THP 1 (Human blood monocyte) | 12-13 | • | • |
| TF-1 | 13-14 | • | • |
| U251 | 16-20 | • | |
| U2OS | 16-19 | • | |
| U266 | 12 | • | |
| U87 (Human Glioblastoma) | 12-14 | • | |
| U937 | 11-13 | • | • |
| WI-38 | 12-15 | • | |
| Y79 | 13-14 | • | |
| Yeast- Pichia Pastoris | 5 | • | |
| Yeast - S. cerevisiae | 6 | • |
Materials
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