LC/MS/MS Analysis of Aflatoxins in Cannabis on Ascentis® Express 2.7µm Phenyl-Hexyl after Cleanup Using Supel™ Tox AflaZea SPE
Figure 1.
Conditions | |
|---|---|
| Sample Preparation | Solid Phase Extraction |
| Sample/Matrix | Cannabis extract spiked with aflatoxins at corresponding concentrations of 6 ppb each (for Aflatoxin B2 and G2) and 24 ppb each (for Afltoxin B1 and G1) in cannabis |
| SPE Tube/Cartridge | Supel Tox AflaZea SPE, 6 mL (55314-U) |
| Sample Addition | 2 mL of sample extract |
| Elution | Apply strong vacuum and collect the sample into plastic tube |
| Eluate Post-treatment | Dilute 0.2 mL of the extract with 0.8 mL distilled water and mix well |
| Column | Ascentis Express Phenyl-Hexyl, 5 cm x 2.1 mm I.D., 2.7 μm particles (53334-U) |
| Column Temp. | 40 °C |
| Mobile Phase | [A] 5 mM ammonium formate with 1% formic acid in water; [B] 5 mM ammonium formate with 1% formic acid in methanol |
| Gradient | 30% B to 60% B in 3 min; to 100% B in 2 in; held at 100% B for 2 min; to 30% B in 0.1 min; held at 30% B for 1.4 min |
| Flow Rate | 0.4 mL/min |
| Pressure | 5511 psi (380 bar) |
| Injection | 10 μL |
| Detector | MS, ESI(+), MRM 331.3/189.0, 329.1/243.0, 315.9/259.0, 313.1/241.0 |
Description | |
|---|---|
| Analysis Note | The complex composition of cannabis makes it difficult to apply sample cleanup methods commonly used for other commodities. In this application, interference removal Supel Tox AflaZea SPE was used for sample cleanup providing quick and effective sample preparation prior to mass spectrometric analysis. Sample comprised 0.5 g of finely ground cannabis mixed with 10 mL of 84:16 acetonitrile:water for 30 minutes and centrifuged. It is recommended to use silanized glass vials for this analysis. |
| Categories | Analytical Chromatography, Drugs of Abuse, Mycotoxins |
| Featured Industry | Food and Beverages Vitamins, Nutraceuticals, and Natural Products |
| Legal Information | Ascentis is a registered trademark of Sigma-Aldrich Co. LLC, Supel is a trademark of Sigma-Aldrich Co. LLC |
| Suitability | Application for LC-MS, application for SPE |
Materials
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