Plant Culture Reagents Applications
Plant cell culture, such as seed culture, meristem culture, callus culture, bud culture, and other plant tissue culture requires specialized supplements and reagents. We offer gelling agents, plant growth regulators, auxins, cytokinins, specialty water and other supplements like antibiotics and amino acids for supporting the growth and development of plant cells in culture.
Products
Plant Tissue Culture Gelling Agents
Plant tissue is cultured in liquid medium or placed on a gel substrate. Agar has long been used as a gelling agent for plant tissue culture. Phytagel™ is an agar substitute produced from a bacterial substrate that is optimized for plant culture. It produces a clear, colorless, high-strength gel, which aids in the detection of microbial contamination. Agargel™ is a blend of agar and Phytagel™ that was developed to help control vitrification in plant tissue cultures, and produces a semi-clear gel that is superior for detecting contamination. Gelrite® (Gelzan™), gelling agent is another agar substitute that can be used in plant tissue culture applications.
Plant Growth Regulators
Plant growth regulators can support cell growth and induce metabolic pathways in plant cell and tissue culture protocols. Plant growth regulators include auxins, cytokinins, and zeatin. Our plant hormone collection includes abscisic, jasmonic, and gibberellic acid. Herbicides validated for plant cell culture include dicamba and glyphosate (Roundup®).
Plant Auxins and Cytokinins
Auxins are used in plant cell culture to promote cell division, cell elongation, and callus formation. Our auxin catalog includes the most commonly used in plant cell culture — 2,4-dichlorophenoxyacetic acid — plus 3-indoleacetic acid, 1-naphthaleneacetic acid, phenylacetic acid, 2,3,5-triiodobenzoic acid, and 2,4,5-trichlorophenoxyacetic acid.
Cytokinins promote cell division and differentiation in plant tissue culture. Kinetin and benzyl-aminopurine are the most commonly used cytokinins in plant cell culture. Our plant culture-approved cytokinins include kinetin, benzylaminopurines, adenines, zeatin, and thidiazuron.
Plant Culture Vitamin Mixes and Other Reagents
Plant tissue culture requires specialized plant culture media and supplements. We offer Gamborg’s vitamin mix, salts, banana powder, antibiotics (neomycin, rifampicin, vancomycin, paromomycin), specialty waters including seawater and coconut water, as well as other vital supplements for optimizing the growth of plant cells and tissues in culture.
Related Resources
- Antibiotics in Plant Tissue Culture Protocol
Reference guide and preparation guide for antibiotic and antimycotic use in plant tissue culture.
- Classical Plant Media Formulations
Classical plant tissue culture media developed years ago by pioneers such as Murashige, Skoog, Gamborg, and others still play a vital role in plant tissue culture research today.
- Explant Sterilization – Plant Tissue Culture Protocol
Surface sterilizing plant material is an important for plant tissue culture. Commonly used disinfectants for plant tissue culture and methods for disinfecting seeds are included.
- Growth Regulators – Plant Tissue Culture Protocol
Use the tables in this protocol to assist in the preparation of auxins, cytokins, and other regulators for use in plant cell culture media.
- Media Preparation – Plant Tissue Culture Protocol
Preparation from Packaged Powder, Preparation from Basal Salt Solutions, Banana Powder Preparation and Use, Coconut Water Preparation and Use
- Pathology Media – Plant Tissue Culture Protocol
Fungal and Bacterial Detection Media, Media Preparation and Storage, Media Composition Table.
- Phycology Media in Plant Tissue Culture
Phycology media can be prepared from concentrated solutions or from powdered salt mixtures. The concentrated solutions are complete, including vitamins, and should be stored frozen.
- Brochure: Plant Tissue Culture Products to Ensure Your Sucess
Our combination of qualified plant tissue culture products and global business capabilities help customers maintain reagent consistency and suitability.
- Silver Thiosulfate – Plant Tissue Culture Protocol
Preparation instructions for 0.1 M Sodium Thiosulfate (STS) and 0.1 M Silver Nitrate stock solutions for laboratory use.
- Vitamin Mixtures – Plant Tissue Culture Protocol
Preparation of Solutions from Packaged Powder, Media Preparation using Vitamin Solutions, Vitamin Mixtures Composition Table
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