G005713
LC/MS Analysis of Omeprazole and Metabolites on Ascentis® Express RP Amide (pH 2.7)
application for HPLC
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express RP-Amide, 5 cm x 2.1 mm I.D., 2.7 μm particles (53911-U)
mobile phase: [A] 10 mM ammonium formate in water, pH 2.7; [B] 10 mM ammonium formate in methanol, pH 2.7; (65:35, A:B)
flow rate: 0.3 mL/min
column temp.: 35 °C
detector: ESI(+), m/z 50-400
injection: 1 μL
sample: 1 mg/L in mobile phase
suitability
application for HPLC
application(s)
clinical
Analysis Note
Legal Information
Analyte
analytical column
mobile phase component
standard
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