Certified Reference Microorganisms
For Reliable Performance Testing
Quality control is an essential element of a laboratory’s quality assurance system and certified reference materials (CRMs) are necessary for effective quality control. The Vitroids™ and LENTICULE® discs from Millipore are specially developed to provide laboratories with the ability to use suitable, ready-to-use microbiological controls for every media quality control test.
The Colony forming unit (cfu)-concentrations are designed ensuring that no or only minimal dilution steps are required and are provided under license by National Collection of Type Cultures (NCTC®)/ National Collection of Pathogenic Fungi (NCPF®) and Spanish Type Culture Collection (Colección Española de Cultivos Tipo, CECT®), respectively.
The LENTICULE® and Vitroids™ discs are manufactured under conditions compliant with ISO 17034 and ISO/IEC 17025. These discs contain defined certified cfu’s with a standard deviation and expanded uncertainty. The discs contain pure cultures of bacteria, yeasts or molds in a solid water-soluble matrix. The certified reference material for microorganisms finds its application in routine quality control for food, beverage, water and environmental testing, for method development and validations and as cultures used in research studies.
Vitroids™ and LENTICULE® discs are readily available as small water-soluble discs, simplifying the inoculum preparation process. You can watch a video demonstrating the step-by-step procedures for using these CRM discs directly or through liquid media before inoculation.
- Key features: Certified CFU, reliable defined inoculum ranges from 15-150000 per discs.
- Stability: Stable for nearly 16 months, can be stored at -20 °C. The numbers of CFUs do not change, the organisms need no recovery time and have no lag phase.
- Time saving: Saves 1 hour per test strain. Discs are time saving because it removes the need for preparing stock cultures. The organisms do not require recovery time or pre-enrichment step.
- Accuracy: Standard deviation and concentration range on Certificate of Quality (CoQ).
- Traceability: The discs are prepared from a traceable culture obtained freeze-dried from CECT®, NCTC® and NCPF® collection. Discs are traceable compared to a CRM for characterization. Each plate is stored as image and all the documents and data are saved as well.
Bacteria standards
We provide an extensive range of pure cultures of bacteria CRMs LENTICULE® and Vitroids™ discs that are stable for at least one year. The discs are supplied with bacteria in a viable stage (no lag phase or recovery time) and a comprehensive certificate of analysis that specifies the mean number of cfu’s, and an expanded uncertainty about the mean value.
Yeast standards
We supply yeast CRMs in Vitroids™ discs with cfu values ranging from 15–80, 80-130 and 1,000-10,000 for your laboratory needs.
Mold standards
We offer mold CRMs in the form of Vitroids™ discs to ensure the safety and efficacy of your products, that are routinely exposed to molds and fungi.
Negative controls standards
We have negative control CRMs in the form of LENTICULE® discs that ensures the effectiveness of your microbiological experiments.
Watch the webinar on Microbiological Certified Reference Material (CRM) to explore the technical requirements and applications of CRMs and the innovative EN ISO 17034:2016 and ISO/IEC 17025:2017 accredited Vitroids™ and LENTICULE® discs.
bacilli
Gammaproteobacteria
Other bacteria
Vitroids™ and LENTICULE® discs save time and costs as it eliminates the need for preparing stock cultures. The organisms do not require recovery time or pre-enrichment steps either.
Learn how to use certified reference materials (CRMs) to perform a spiking protocol for method verification.
Read the frequently asked questions about Vitroids™ and LENTICULE® CRMs from the experts
Our culture media is in compliance with ISO 11133 and the corresponding QC strains - available as ready-to-use Vitroids™ and LENTICULE® discs ;
Looking to simplify your culture media performance testing? Our Vitroids™ and LENTICULE® Discs Certified Reference Materials are tailored to streamline your growth promotion tests. They offer ready-to-use discs with practical CFU levels.
All the strains for the Vitroids™ and LENTICULE® discs are sourced from the cell culture collections. You can read the whitepaper to discover the history of each strain used, including gene sequences and cross-references to ATCC, CMCC, and other cell culture collections.
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